您好,欢迎访问中国农业科学院 机构知识库!

Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings

文献类型: 外文期刊

作者: Ma Qiao-li 1 ; Kang Jun-mei 2 ; Long Rui-cai 2 ; Cui Yan-jun 2 ; Zhang Tie-jun 2 ; Xiong Jun-bo 2 ; Yang Qing-chuan 2 ; S 1 ;

作者机构: 1.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

3.Ningxia Univ, Sch Chem & Chem Engn, Yinchuan 750021, Peoples R China

4.Hubei Acad Agr Sci, Inst Anim Sci, Wuhan 430064, Peoples R China

关键词: alfalfa;salinity stress;osmotic stress;seedling growth;proteomics

期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )

ISSN: 2095-3119

年卷期: 2016 年 15 卷 10 期

页码:

收录情况: SCI

摘要: Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mechanisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L-1 NaCl and 180 g L-1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statistically significant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identification, owing to their good resolution and abundance levels, and 32 proteins were positively identified. The identified proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins will provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.

  • 相关文献

[1]Isolation and characterization of GoDREB encoding an ERF-type protein in forage legume Galegae orientalis. Wang, Xuemin,Chen, Xiaofang,Wang, Zan,Gao, Hongwen,Nikolay, Dzyubenko,Vladimir, Chapurin.

[2]A Novel Dehydration-Responsive Element-Binding Protein from Caragana korshinskii Is Involved in the Response to Multiple Abiotic Stresses and Enhances Stress Tolerance in Transgenic Tobacco. Wang, Xuemin,Dong, Jie,Gao, Hongwen,Liu, Yun.

[3]Proteomic response of oat leaves to long-term salinity stress. Bai, Jianhui,Qin, Yan,Wang, Yuqing,Bai, Jianhui,Liu, Jinghui,Sa, Rula,Zhang, Na,Jia, Ruizong.

[4]Proteomic Analysis of Osmotic Stress-Responsive Proteins in Sugarcane Leaves. Li, Yang-Rui,Qiu, Li-Hang,Huang, Xing,Zhou, Gui,Zou, Cheng-Lin,Huang, Li-Ping,Li, Yang-Rui,Srivastava, Manoj Kumar.

[5]Comparative proteomic analysis of alfalfa revealed new salt and drought stress-related factors involved in seed germination. Ma, Qiaoli,Kang, Junmei,Zhang, Kun,Wang, Tenghua,Sun, Yan,Kang, Junmei,Long, Ruicai,Zhang, Tiejun,Yang, Qingchuan,Ma, Qiaoli,Xiong, Junbo.

[6]卫星搭载对紫花苜蓿当代植株生物量的影响(英文). 任卫波,赵亮,王蜜,陈立波,郭慧琴. 2008

[7]HYDROGEL DISCS ON DIGITAL MICROFLUIDIC DEVICES FOR PROTEOMIC APPLICATIONS. Vivienne N. Luk,Lindsey K. Fiddes,Alphonsus H.C. Ng,Eugenia Kumacheva,Aaron R. Wheeler. 2011

[8]BLOOD PLASMA PROTEOME AND PEPTIDOME COMPARATIVE SCREENING FOR POTENTIAL BIOMARKERS OF ALZHEIMER'S DISEASE. Maria Indeykina,Anna Bugrova,Natalia Zakharova,Alexey Kononikhin,Yana Fedorova,Alexander Brhozovskiy,Igor Popov,Svetlana Gavrilova,Evgeny Nikolaev. 2018

[9]Allelopathic effects of aerial parts of Descurainia sophia L. on wheat. Liu, X. G.,Lu, C. H.,Dong, F. S.,Xu, J.,Wu, Y. B.,Zheng, Y. Q.,Tian, F. J.,Wu, Y. B.. 2016

[10]Overexpression of a Wheat CCaMK Gene Reduces ABA Sensitivity of Arabidopsis thaliana During Seed Germination and Seedling Growth. Yang, Cui,Kang, Zhensheng,Yang, Cui,Kang, Zhensheng,Yang, Cui,Li, Aili,Zhang, Zenglin,Zhu, Yuanfang,Tan, Xiaomei,Guo, Hanzi,Zhang, Xueyong,Mao, Long,Zhao, Yongliang,Geng, Shuaifeng.

[11]AtPPR2, an Arabidopsis pentatricopeptide repeat protein, binds to plastid 23S rRNA and plays an important role in the first mitotic division during gametogenesis and in cell proliferation during embryogenesis. Lu, Yuqing,Li, Cong,Lu, Yuqing,Wang, Hai,Chen, Hao,Berg, Howard,Xia, Yiji,Lu, Yuqing,Wang, Hai,Xia, Yiji.

[12]Molecular cloning and characterization of a salinity stress-induced gene encoding DEAD-box helicase from the halophyte Apocynum venetum. H. H. Liu,J. Liu,S. L. Fan,M. Z. Song,X. L. Han,F. Liu,F. F. Shen.

[13]Responses of antioxidant gene, protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance. Hu, Longxing,Li, Huiying,Fu, Jinmin,Pang, Huangcheng. 2012

[14]Growth and physiological response of tall oat grass to salinity stress. Wang Zan,Yang Xi,Wang Xue-Min,Gao Hong-Wen. 2011

[15]PHYSIOLOGICAL AND BIOCHEMICAL RESPONSES TO NACl SALINITY STRESS IN THREE ROEGNERIA (POACEAE) SPECIES. Xie, Jihong,Dai, Yating,Mu, Huaibin,De, Ying,Wu, Zinian,Yu, Linqing,Ren, Weibo,Xie, Jihong,Dai, Yating,Mu, Huaibin,De, Ying,Wu, Zinian,Yu, Linqing,Ren, Weibo,Chen, Hao. 2016

[16]Diversity in D-genome synthetic hexaploid wheat association panel for seedling emergence traits under salinity stress. Khan, Zeeshan,Qazi, Javaria,Rasheed, Awais,Rasheed, Awais,Mujeeb-Kazi, Abdul.

[17]Physiological effects of exogenous nitric oxide on Brassica juncea seedlings under NaCl stress. Zeng, C. -L.,Zhou, Y.,Zeng, C. -L.,Wu, X. -M.,Liu, L.,Wang, B. -R..

[18]Analysis of the Role of the Drought-Induced Gene DRI15 and Salinity-Induced Gene SI1 in Alternanthera philoxeroides Plasticity Using a Virus-Based Gene Silencing Tool. Bai, Chao,Wang, Peng,Fan, Qiang,Wang, Le,Zhang, Zhen-Nan,Wu, Jia-He,Bai, Chao,Fu, Wei-Dong,Song, Zhen,Zhang, Guo-Liang. 2017

[19]Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar. Chen, Guang,Liu, Chaolei,Gao, Zhenyu,Zhang, Yu,Zhang, Anpeng,Zhu, Li,Hu, Jiang,Ren, Deyong,Qian, Qian,Chen, Guang,Yu, Ling,Xu, Guohua. 2018

[20]Strigolactones Improve Plant Growth, Photosynthesis, and Alleviate Oxidative Stress under Salinity in Rapeseed (Brassica napus L.) by Regulating Gene Expression. Ma, Ni,Hu, Chao,Wan, Lin,Hu, Qiong,Xiong, Junlan,Zhang, Chunlei,Ma, Ni,Zhang, Chunlei,Hu, Chao. 2017

作者其他论文 更多>>