Copper chelating peptides derived from tilapia (Oreochromis niloticus) skin as tyrosinase inhibitor: Biological evaluation, in silico investigation and in vivo effects
文献类型： 外文期刊
作者： Song, Yuqiong 1 ; Li, Jun 1 ; Tian, Han 3 ; Xiang, Huan 1 ; Chen, Shengjun 1 ; Li, Laihao 1 ; Hu, Xiao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Aquat Prod Proc, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China
2.Chinese Acad Sci, RNAM Ctr Marine Microbiol South China Sea Inst Oce, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China
3.Shanghai Ocean Univ, Coll Marine Sci, Shanghai 201306, Peoples R China
4.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
5.Dalian Polytech Univ, Collaborat Innovat Ctr Prov & Ministerial Coconstr, Dalian 116034, Peoples R China
关键词： Tilapia (Oreochromis niloticus) skin; Copper chelating peptides; Tyrosinase inhibition; Molecular dynamics
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）
ISSN： 0963-9969
年卷期： 2023 年 163 卷
页码：
收录情况： SCI
摘要： Binuclear copper ions at the active site determine the catalysis of tyrosinase (TYR)1 whose activity can be inhibited by copper's chelation with other compounds. In this study, tilapia (Oreochromis niloticus) skin was used to generate TYR-inhibitory peptides after being treated by different enzymes and 4 h-Alcaline protease hydrolysate exhibited the highest TYR inhibition and copper chelation. Immobilized metal affinity chromatog-raphy was used for purifying copper chelating peptides, among which PFRMY (IC50: 0.43 +/- 0.08 mg/mL) and RGFTGM (IC50: 1.61 +/- 0.04 mg/mL) exhibited the highest TYR-inhibitory capacity and the lowest docking energy. Both two peptides inhibited TYR in a mixed manner and interacted with key residues binding to copper ions within TYR mainly by hydrogen bonds and hydrophobic forces, while PFRMY had a more compact and stable conjugation with TYR. Zebrafish assay revealed that PFRMY reduced not only melanin synthesis but in vivo TYR activity.
- 相关文献
作者其他论文 更多>>
-
Emulsification properties and oil-water interface properties of L-lysine-assisted ultrasonic treatment in sea bass myofibrillar proteins: Influenced by the conformation of interfacial proteins
作者：Wu, Yamei;Wu, Yanyan;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Wang, Yueqi;Wu, Yanyan;Cai, Qiuxing;Wang, Yueqi;Wu, Yamei;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Wang, Yueqi;Wu, Yamei;Chen, Shengjun;Zhao, Yongqiang;Wu, Yanyan;Chen, Shengjun;Zhao, Yongqiang;Wang, Yueqi;Wu, Yanyan;Wang, Yueqi
关键词：Sea bass; Myofibrillar protein; Protein conformation; Interfacial properties; Emulsification properties
-
Insights into microbiota community dynamics and flavor development mechanism during golden pomfret (Trachinotus ovatus) fermentation based on single-molecule real-time sequencing and molecular networking analysis
作者：Wang, Yueqi;Chen, Qian;Xiang, Huan;Sun-Waterhouse, Dongxiao;Chen, Shengjun;Zhao, Yongqiang;Li, Laihao;Wu, Yanyan;Wang, Yueqi;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Li, Laihao;Wu, Yanyan;Sun-Waterhouse, Dongxiao;Wang, Yueqi;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Li, Laihao;Wu, Yanyan
关键词：Fermented golden pomfret; Microbiota community; Volatile compound; Co-occurrence network; Metabolic pathway
-
Unraveling the antibacterial mechanism of Lactiplantibacillus plantarum MY2 cell-free supernatants against Aeromonas hydrophila ST3 and potential application in raw tuna
作者：Wang, Di;Liu, Yangfan;Chen, Shengjun;Deng, Jianchao;Li, Chunsheng;Pan, Chuang;Wang, Yueqi;Xiang, Huan;Feng, Yang;Yang, Xianqing;Li, Laihao;Wu, Yanyan;Zhao, Yongqiang;Qi, Bo;Wei, Ya;Wang, Di;Chen, Shengjun;Deng, Jianchao;Li, Chunsheng;Pan, Chuang;Wang, Yueqi;Xiang, Huan;Feng, Yang;Yang, Xianqing;Li, Laihao;Wu, Yanyan;Zhao, Yongqiang;Qi, Bo;Wei, Ya;Wang, Di;Chen, Shengjun;Deng, Jianchao;Wang, Di;Chen, Shengjun;Deng, Jianchao;Li, Chunsheng;Pan, Chuang;Wang, Yueqi;Xiang, Huan;Feng, Yang;Yang, Xianqing;Li, Laihao;Wu, Yanyan;Zhao, Yongqiang;Qi, Bo;Wei, Ya;Li, Xupeng;Chen, Shengjun
关键词：Aeromonas hydrophila; Lactiplantibacillus plantarum; Cell free supernatants; Antibacterial; Metabolomics
-
Effects of konjac glucomannan on physical properties and microstructure of fish myofibrillar protein gel: Phase behaviours involved
作者：Zhang, Tao;Zhuang, Xinbo;Chen, Yinji;Chen, Shengjun;Xu, Xiaoqi;Xue, Yong;Xue, Changhu;Jiang, Ning
关键词：Phase behavior; Dynamic rheology; Myofibrillar protein; Konjac glucomannan; Blended system
-
Diversity and Carbon Sequestration of Seaweed in the Ma'an Archipelago, China
作者：Li, Xunmeng;Zhao, Xu;Zhang, Shouyu;Chen, Jianqu;Wang, Zhenhua;Wang, Kai;Yuan, Huarong;Guo, Yu;Li, Jun
关键词：seaweed; Ma'an Archipelago; diversity; distribution; carbon sequestration
-
Proteomics study of mitochondrial proteins in tilapia red meat and their effect on color change during storage
作者：Xiang, Huan;Sun, Shenyu;Huang, Hui;Hao, Shuxian;Li, Laihao;Yang, Xianqing;Chen, Shengjun;Wei, Ya;Cen, Jianwei;Pan, Chuang;Xiang, Huan;Huang, Hui;Hao, Shuxian;Li, Laihao;Yang, Xianqing;Chen, Shengjun;Wei, Ya;Cen, Jianwei;Pan, Chuang;Xiang, Huan;Huang, Hui;Hao, Shuxian;Li, Laihao;Yang, Xianqing;Chen, Shengjun;Wei, Ya;Cen, Jianwei;Pan, Chuang
关键词：Tilapia; Label -free; Mitochondrial proteins; Color changing; TCA cycle
-
Characterization of the aggregation behavior of sea bass (Lateolabrax japonicus) myofibrillar proteins mediated by different ionic strengths: Protein structures, gel properties, and emulsion stabilities
作者：Wu, Yamei;Xiang, Huan;Chen, Shengjun;Zhao, Yongqiang;Lin, Wanling;Wu, Yanyan;Wang, Yueqi;Cai, Qiuxing;Wu, Yanyan;Wang, Yueqi;Cai, Qiuxing;Wu, Yanyan;Wang, Yueqi;Wu, Yamei;Wu, Yamei;Chen, Shengjun;Zhao, Yongqiang;Wu, Yanyan;Wang, Yueqi;Chen, Shengjun;Zhao, Yongqiang;Wu, Yanyan;Wang, Yueqi
关键词：Lateolabrax japonicus; Emulsifying properties; Gelation; Structural properties; Functional properties
