文献类型： 外文期刊
作者： Li, Junlin 1 ; Li, Zhifei 2 ; Xie, Jun 2 ; Xia, Yun 2 ; Gong, Wangbao 2 ; Tian, Jingjing 2 ; Zhang, Kai 2 ; Yu, Ermeng 2 ; Wang, Guangjun 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, 999 Huchenghuan Rd, Shanghai, Peoples R China
2.Chinese Acad Fishery Sci CAFS, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat &, 1 Xingyu Rd, Guangzhou 510380, Peoples R China
3.Guangdong Ecol Remediat Aquaculture Pollut Res Ctr, Guangzhou 510380, Peoples R China
关键词： Quorum sensing; Quorum quenching; Biofilm; Bacteria; Aquaculture
期刊名称：INTERNATIONAL MICROBIOLOGY （ 影响因子：3.1； 五年影响因子：3.2 ）
ISSN： 1139-6709
年卷期： 2023 年 26 卷 3 期
页码：
收录情况： SCI
摘要： Quorum sensing (QS) is a core mechanism for bacteria to regulate biofilm formation, and therefore, QS inhibition or quorum quenching (QQ) is used as an effective and economically feasible strategy against biofilms. In this study, the PvdQ gene encoding AHL acylase was introduced into Escherichia coli (DE3), and a PvdQ-engineered bacterium with highly efficient QQ activity was obtained and used to inhibit biofilm formation. Gene sequencing and western blot analysis showed that the recombinant pET-PvdQ strain was successfully constructed. The color reaction of Agrobacterium tumefaciens A136 indicated that PvdQ engineering bacteria had shown strong AHL signal molecule quenching activity and significantly inhibited the adhesion (motility) of Pseudomonas aeruginosa and biofilm formation of activated sludge bacteria in Membrane Bio-Reactor (MBR; inhibition rate 51-85%, p < 0.05). In addition, qRT-PCR testing revealed that recombinant PvdQ acylase significantly reduced the transcription level of QS biofilm formation-related genes (cdrA, pqsA, and lasR; p < 0.05). In this study, QQ genetically engineered bacteria enhanced by genetic engineering could effectively inhibit the QS signal transduction mechanism and have the potential to control biofilm formation of pathogenic bacteria in the aquaculture environment, providing an environmentally friendly and alternative antibiotic strategy to suppress biofilm contamination.
- 相关文献
作者其他论文 更多>>
-
Community assembly patterns and processes of bacteria in a field-scale aquaculture wastewater treatment system
作者：Niu, Shuhui;Xie, Jun;Wang, Guangjun;Li, Zhifei;Zhang, Kai;Li, Hongyan;Xia, Yun;Tian, Jingjing;Yu, Ermeng;Xie, Wenping;Gong, Wangbao;Niu, Shuhui;Xie, Jun;Wang, Guangjun;Li, Zhifei;Zhang, Kai;Li, Hongyan;Xia, Yun;Tian, Jingjing;Yu, Ermeng;Xie, Wenping;Gong, Wangbao;Xie, Jun;Wang, Guangjun;Li, Zhifei;Zhang, Kai;Li, Hongyan;Xia, Yun;Tian, Jingjing;Yu, Ermeng;Xie, Wenping;Gong, Wangbao;Gong, Wangbao
关键词：Field -scale aquaculture wastewater treatment system; Microbial diversity; Stochastic processes; Network complexity
-
Iron-based multi-carbon composite and Pseudomonas furukawaii ZS1 co-affect nitrogen removal, microbial community dynamics and metabolism pathways in low-temperature aquaculture wastewater
作者：Lu, Zhuoyin;Cheng, Xiangju;Li, Zhifei;Zhu, Dantong;Xie, Jun;Li, Zhifei;Li, Xiangyang;Jiang, Xiaotian;Li, Xiangyang
关键词：Low temperature; Aquaculture wastewater; Nitrogen removal; Iron-based multi-carbon source composite; Aerobic denitrifier; Metagenome
-
Dietary deoxycholic acid decreases fat accumulation by activating liver farnesoid X receptor in grass crap (Ctenopharyngodon idella)
作者：Du, Yihui;Wang, Guangjun;Yu, Ermeng;Xie, Jun;Xia, Yun;Li, Hongyan;Zhang, Kai;Gong, Wangbao;Li, Zhifei;Xie, Wenping;Jiang, Peng;Tian, Jingjing;Zhang, Wen;Shao, Li
关键词：Bile acids; Fatty liver; Aquaculture; Feed additive; Gut-liver axis
-
The effect of broad bean diet on structure, flavor and taste of fresh grass carp: A comprehensive study using E-nose, E-tongue, TPA, HS-SPME-GC-MS and LC-MS
作者：Fu, Bing;Yang, Huici;Li, Yichao;Wang, Guangjun;Tian, Jingjing;Zhang, Kai;Xia, Yun;Li, Zhifei;Gong, Wangbao;Li, Hongyan;Xie, Jun;Yu, Ermeng;Fu, Bing;Zheng, Mengping;Yang, Huirong;Fu, Bing;Zheng, Mengping;Yang, Huirong;Zhang, Junming
关键词：Broad bean; Ctenopharyngodon idellus; Flavor; Taste; PLS-SEM
-
The effect of nitrite and nitrate treatment on growth performance, nutritional composition and flavor-associated metabolites of grass carp (Ctenopharyngodon idella)
作者：Zhang, Jun -Ming;Fu, Bing;Li, Yi-chao;Xie, Jun;Wang, Guang-Jun;Tian, Jing-Jing;Kaneko, Gen;Yu, Er-Meng;Zhang, Jun -Ming;Sun, Jin-Hui;Kaneko, Gen;Fu, Bing
关键词：Nitrite; Flavor; Grass carp; Metabolomics
-
Do ectoparasites on fish gills ?talk? with gut microbiota far away?
作者：Wang, Lin;Zhang, Defeng;Xie, Jun;Chang, Ouqin;Wang, Qing;Shi, Cunbin;Zhao, Fei;Gong, Hua;Ren, Yan;Pan, Houjun;Wang, Lin;Xie, Jun;Pan, Houjun;Musa, Najiah;Lee, Kok Leong;Xie, Jun;Pan, Houjun
关键词：Dactylogyrus lamellatus; Cetobacterium; Bacteroides; GPX; Grass carp
-
Hif1 & alpha;/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-& gamma; in Fish Hepatocytes
作者：Tian, Jingjing;Du, Yihui;Wang, Binbin;Ji, Mengmeng;Li, Hongyan;Xia, Yun;Zhang, Kai;Li, Zhifei;Xie, Wenping;Gong, Wangbao;Yu, Ermeng;Wang, Guangjun;Xie, Jun;Tian, Jingjing;Du, Yihui;Wang, Binbin;Ji, Mengmeng;Li, Hongyan;Xia, Yun;Zhang, Kai;Li, Zhifei;Xie, Wenping;Gong, Wangbao;Yu, Ermeng;Wang, Guangjun;Xie, Jun;Tian, Jingjing;Li, Hongyan;Xia, Yun;Zhang, Kai;Li, Zhifei;Xie, Wenping;Gong, Wangbao;Yu, Ermeng;Wang, Guangjun;Xie, Jun
关键词：aquaculture; fatty liver; fatty acid; hypoxia; cell culture; lipid droplet; lipoprotein; liver; retinoids
