IDENTIFICATION OF A SPECIFIC SCAR MARKER FOR DETECTION OF Tilletia foetida (Wall) Liro PATHOGEN OF WHEAT
文献类型： 外文期刊
作者： Zhang, M. 1 ; Chen, W. Q. 2 ; Liu, D. 1 ; Liu, T. G. 2 ; Gao, L. 2 ; Shu, K. 1 ;
作者机构： 1.Sichuan Agr Univ, Dept Plant Pathol, Yaan 625014, Sichuan, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
期刊名称：RUSSIAN JOURNAL OF GENETICS （ 影响因子：0.581； 五年影响因子：0.729 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Common bunt is one of the most important destructive diseases of wheat worldwide and is a domestic quarantined disease in China. However, a rapid and efficient method to identify the corresponding pathogens is currently limited The objective of the present study was to develop a diagnostic molecular marker specific towards Tilletia foetida (Wall) Liro, a causal agent of the bunt disease. One specific DNA fragment for T. foetida (286 bp in length) was amplified using an Amplified Fragment Length Polymorphism (AFLP) assay and this fragment was cloned and sequenced. One pair of specific primers (SC_(286_1)/SC_(286.2)), which was designed according to the sequence, could specifically amplify the corresponding fragment in all of the T. foetidaf isolatesemployed from both the People's Republic of China and United States, whereas this fragment could not be amplified by the other fungal species tested. Therefore, a specific Sequence Characterized Amplified Region (SCAR) marker was developed. This SCAR marker could distinguish T. foetida from related pathogenic fungi efficiently and could be used for the early diagnosis of the common bunt of wheat in the field, and provide an efficient way for disease surveillance and disease forecasting in cereal crop.
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