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INFLUENCE OF RNAi KNOCKDOWN FOR E-COMPLEX GENES ON THE SILKWORM PROLEG DEVELOPMENT

文献类型: 外文期刊

作者: Xiang, H. 1 ; Li, M. W. 1 ; Guo, J. H. 1 ; Jiang, J. H. 1 ; Huang, Y. P. 1 ;

作者机构: 1.Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang 212018, Peoples R China

关键词: RNAi;silkworm;proleg;E-complex genes;abd-A

期刊名称:ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:1.698; 五年影响因子:1.758 )

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收录情况: SCI

摘要: Larvae of many holometabolous insects possess abdominal appendages called prolegs. Lepidoptera larvae have prolegs in the segments A3–A6. Functions of Lepidoptera hox genes on these abdominal appendages development is still a controversial issue. Inthis article, we report the use of double strand RNA (dsRNA)-mediated interference (RNAi) to dissect the function of some hox genes, specifically E-complex genes Ubx, abd-A, and Abd-B, in the ventral appendage development of the Lepidoptera silkworm, Bombyx mori. We found that Ubx RNAi caused leg identity in A1 segment, abd-A RNAi caused severe defect of abdominal prolegs and Abd-B RNAi allowed proleg identity in more posterior abdominal segments. These results confirm that Lepidoptera hox genes Ubx andAbd-B have evolved the repressing function to ventral appendage development, which is similar to those of Drosophila. However, Lepidoptera abd-A might have been modified distinctively during evolution, and has important roles in directing the development of prolegs.

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