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Amycolatopsis melonis sp. nov., a novel protease- producing and cellulose- degrading actinobacterium isolated from soil

文献类型： 外文期刊

作者： Xu, Yan 1 ; Zhao, Xue 1 ; Jin, Jiaxin 1 ; Zhang, Ran 1 ; Zhou, Changjian 1 ; Wang, Zishan 1 ; Yao, Siqi 1 ; Wang, Xiangjing 1 ; Xiang, Wensheng 1 ; Song, Jia 1 ;

作者机构： 1.Northeast Agr Univ, Key Lab Agr Microbiol Heilongjiang Prov, Harbin 150030, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China

关键词： 16S rRNA gene; genome characterization; novel species; taxonomy

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.0； 五年影响因子：2.2 ）

ISSN： 1466-5026

年卷期： 2024 年 74 卷 10 期

页码：

收录情况： SCI

摘要： A novel protease- producing and cellulose- degrading actinobacterium, designated strain NEAU- NG30T, was isolated from a melon rhizosphere soil sample collected in Harbin, Heilongjiang Province, China, and established its status using a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NEAU-NG30T was closely related to Amycolatopsis bullii DSM 45802T (98.7%) and Amycolatopsis vancoresmycina DSM 44592T (98.3%). The phospholipid profile contained diphosphatidylglycerol, phosphatidyl methylethanolamine, phosphatidylethanolamine and phosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be galactose and arabinose. Cell walls contained meso- diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK- 9(H4). The major fatty acids were iso-C15:0 and iso- C 16:0 . Meanwhile, genome analysis of sequences revealed a genome size of 9 338 250 bp and a DNA G+C content of 71.6%. In addition, the average nucleotide identity values and the level of digital DNA-DNA hybridization between strain NEAU-NG30T and its reference strains fall below the thresholds typically used for delineating prokaryote species. According to phenotypic, chemotaxonomic and genotypic studies, it is indicated that strain NEAU-NG30T is considered to be a novel species of the genus Amycolatopsis, for which the name Amycolatopsis melonis sp. nov. is proposed, with NEAU-NG30T (=MCCC 1K08677T=JCM 35654T) as the type strain.

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