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Transcription factors PuNAC37/PuWRKY74 and E3 ubiquitin ligase PuRDUF2 inhibit volatile ester synthesis in 'Nanguo' pear

文献类型： 外文期刊

作者： Zang, Nannan 1 ; Li, Xiaojing 1 ; Zhang, Zhuoran 1 ; Liu, Weiting 1 ; Qi, Liyong 1 ; Yang, Yueming 1 ; Sun, Qitian 1 ; Yin, Zepeng 1 ; Wang, Aide 1 ;

作者机构： 1.Shenyang Agr Univ, Key Lab Fruit Postharvest Biol Liaoning Prov, Coll Hort, Shenyang 110866, Peoples R China

2.Natl & Local Joint Engn Res Ctr Northern Hort Faci, Key Lab Protected Hort, Shenyang 110866, Peoples R China

3.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China

4.Chinese Acad Agr Sci, Res Inst Pomol, Xinghai South St 98, Xingcheng 125100, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：6.9； 五年影响因子：7.7 ）

ISSN： 0032-0889

年卷期： 2024 年 197 卷 1 期

页码：

收录情况： SCI

摘要： As major contributors to flavor in many fruit species, volatile esters are useful for investigating the regulation of the biosynthesis pathways of volatile aroma compounds in plants. Ethylene promotes the biosynthesis of volatile esters during fruit storage while accelerating fruit ripening; thus, the ethylene perception inhibitor 1-methylcyclopropene (1-MCP) is employed to prolong the shelf life of fruits. However, the mechanisms by which 1-MCP regulates volatiles synthesis remain unclear. In this study, we analyzed the pathway of 1-MCP-mediated volatile ester synthesis in 'Nanguo' pear (Pyrus ussuriensis). 1-MCP significantly decreased volatile ester synthesis during storage. Comparative transcriptome analysis showed that the genes encoding two transcription factors (PuNAC37 and PuWRKY74) and a RING-type E3 ubiquitin ligase that have a domain of unknown function (PuRDUF2) were expressed at high levels, whereas ALCOHOL ACYLTRANSFERASE 1 (PuAAT1), encoding an enzyme responsible for volatile ester synthesis, was expressed at low levels in 1-MCP-treated fruit. Moreover, PuNAC37 inhibited the expression of PuWRKY74 via transcriptional regulation, whereas PuNAC37 and PuWRKY74, after directly binding to the promoter of PuAAT1, synergistically inhibited its expression in 1-MCP-treated fruit. In addition, in vitro and in vivo ubiquitination experiments revealed that PuRDUF2 functions as an E3 ubiquitin ligase that ubiquitinates PuAAT1, causing its degradation via the 26S proteasome pathway following 1-MCP treatment. Subsequent PuAAT1 degradation resulted in a reduction of volatile esters during fruit storage. Our findings provide insights into the complex transcriptional regulation of volatile ester formation in 'Nanguo' pears and contribute to future research on AAT protein ubiquitination in other species. Two transcription factors and an ubiquitin ligase reduce the accumulation of volatile ester aroma, resulting in a decline in the fruit aroma quality.

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