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OsKANADI1 and OsYABBY5 regulate rice plant height by targeting GIBERELLIN 2-OXIDASE6

文献类型: 外文期刊

作者: He, Qi 1 ; Wu, Hao 2 ; Zeng, Longjun 3 ; Yin, Caiyun 1 ; Wang, Li 6 ; Tan, Yiqing 7 ; Lv, Wanqing 7 ; Liao, Zhiqiang 3 ; Zheng, Xuelian 8 ; Zhang, Shuting 8 ; Han, Qinqin 8 ; Wang, Danning 8 ; Zhang, Yong 2 ; Xiong, Guosheng 7 ; Wang, Quan 1 ;

作者机构: 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Minist Agr & Rural Affairs, Guangdong Lab Lingnan Modern Agr,Genome Anal Lab, Shenzhen 518120, Peoples R China

2.Southwest Univ, Integrat Sci Ctr Germplasm Creat Western China Cho, Sch Life Sci, Chongqing Key Lab Plant Resource Conservat & Germp, Chongqing 400715, Peoples R China

3.Yichun Acad Sci, Inst Crop Sci, Yichun 336000, Peoples R China

4.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475001, Peoples R China

5.Henan Univ, Shenzhen Res Inst, Shenzhen 518000, Peoples R China

6.Chinese Acad Agr Sci, Inst Cotton Res, Anyang 455000, Peoples R China

7.Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Plant Phen Res Ctr, Nanjing 210095, Peoples R China

8.Univ Elect Sci & Technol China, Ctr Informat Biol, Sch Life Sci & Technol, Dept Biotechnol, Chengdu 611731, Peoples R China

期刊名称:PLANT CELL ( 影响因子:11.6; 五年影响因子:12.1 )

ISSN: 1040-4651

年卷期: 2024 年 37 卷 1 期

页码:

收录情况: SCI

摘要: Plant height is an important agronomic characteristic of rice ( Oryza sativa L. ). Map-based cloning analyses of a natural semi-dwarf rice mutant with inwardly curled leaves found in the field revealed that the defects were due to a mutation of a SHAQKYF-class MYB family transcription factor, OsKANADI1 (OsKAN1). OsKAN1 directly bound to the OsYABBY5 ( OsYAB5 ) promoter to repress its expression and interacted with OsYAB5 to form a functional OsKAN1-OsYAB5 complex. GIBERELLIN 2-OXIDASE6 ( OsGA2ox6 ), encoding an enzyme in the gibberellin (GA) catabolic pathway, was activated by OsYAB5. Furthermore, the OsKAN1-OsYAB5 complex suppressed the inhibitory effect of OsKAN1 toward OsYAB5 and inhibited OsYAB5-induced OsGA2ox6 expression. The proOsKAN1: OsYAB5 transgenic plants were taller than wild-type plants, whereas oskan1 proOsKAN1:OsYAB5 plants exhibited a severe dwarf phenotype due to the absence of the OsKAN1-OsYAB5 complex. The OsKAN1-OsYAB5 complex modulated OsGA2ox6 expression, thereby regulating the levels of bioactive gibberellins and, consequently, plant height. This study elucidated the mechanism underlying the effect of the OsKAN1-OsYAB5-OsGA2ox6 regulatory pathway on plant height at different positions in rice stems and provided insights on stem development and candidate genes for the aerial architecture improvement of crop plants.

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