Sensitive detection of Vibrio parahaemolyticus via a dual-recognition colorimetric biosensor comprising Cefe-PEG-MNPs and apt-Fe@PDA
文献类型： 外文期刊
作者： Lin, Hongyong 1 ; Liao, Tengyang 2 ; Sang, Yaoyao 1 ; Mao, Jin 3 ; Liu, Yu 1 ; Zhang, Bin 2 ; Sun, Di 1 ; Jiang, Wei 1 ;
作者机构： 1.Zhejiang Ocean Univ, Natl Engn Res Ctr Marine Aquaculture, Zhoushan 316022, Peoples R China
2.Zhejiang Ocean Univ, Coll Food & Pharm, Key Lab Key Tech Factors Zhejiang Seafood Hlth Haz, Zhoushan 316022, Peoples R China
3.Chinese Acad Agr Sci, Natl Reference Lab Agr Testing Biotoxin, Lab Qual & Safety Risk Assessment Oilseed Prod Wuh, Key Lab Detect Mycotoxins,Qual Inspect & Test Ctr, Wuhan 430062, Peoples R China
关键词： Vibrio parahaemolyticus; Cefe-PEG-MNPs; Aptamer; Fe@PDA nanozyme; Colorimetric detection
期刊名称：FOOD CONTROL （ 影响因子：6.3； 五年影响因子：6.1 ）
ISSN： 0956-7135
年卷期： 2025 年 168 卷
页码：
收录情况： SCI
摘要： Highly accurate and sensitive detection of Vibrio parahaemolyticus is crucial for ensuring seafood safety and preventing the transmission of foodborne diseases. Herein, a dual-recognition strategy-driven colorimetric biosensor for the detection of V. parahaemolyticus was developed. This biosensor was based on the use of PEG- mediated cefepime-functionalized magnetic nanoparticles (Cefe-PEG-MNPs) and aptamer-modified Fe-doped polydopamine (Fe@PDA) nanozymes. The magnetic platform Cefe-PEG-MNPs was introduced to enhance the enrichment efficiency of V. parahaemolyticus, achieving a capture efficiency exceeding 90%. The Fe@PDA nanozyme exhibited notable peroxidase mimetic enzyme activity. The colorimetric biosensor developed for V. parahaemolyticus detection demonstrated good sensitivity, with a detection range spanning from 2.1 x 101 to 2.1 x 106 CFU/mL, and a limit of detection of 21 CFU/mL. Moreover, the biosensor demonstrated remarkable specificity, achieving good recovery rates from 95.80% to 103.80% in Penaeus vannamei with a relative standard deviation of less than 1.38%. The newly developed colorimetric biosensor offers a remarkable opportunity for the accurate and sensitive detection of V . parahaemolyticus in seafood.
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