文献类型： 外文期刊

作者： Chen, Ying ¹ ; Luo, Chengzeng ¹ ; Zhan, Zhaohan ¹ ; Liu, Shuo ¹ ; Teng, Chunran ¹ ; Mao, Ruixiao ¹ ; Zhang, Shunfen ¹ ; Zhang, Xunbozan ¹ ; Meng, Qingshi ¹ ; Zhong, Ruqing ¹ ; Chen, Liang ¹ ; Zhang, Hongfu ¹ ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Anim Nutr & Feeding, Inst Anim Sci, Beijing 100193, Peoples R China

2.Shanxi Agr Univ, Coll Anim Sci, Jinzhong 030801, Peoples R China

3.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Peoples R China

关键词： Intestinal inflammation; L. reuteri SXDT-32; PI3K-Akt pathway; Shikimic acid

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.5； 五年影响因子：7.2 ）

ISSN： 1674-9782

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Background Colitis caused by bacterial infection is a major global health challenge. Unfortunately, current treatment options are limited. We previously disclosed that L. reuteri SXDT-32 was enriched in the feces of an ancient diarrhea-resistant pig breed (Mashen pig) in China over 2500 years old. As diarrhea is often closely associated with intestinal inflammation, L. reuteri SXDT-32 was identified as a potential beneficial bacterium to prevent intestinal inflammation. However, the precise mechanisms involved remained unclear. Results Our tests showed that L. reuteri SXDT-32 alleviated colonic damage induced by pathogenic E. coli SKLAN202302 in weaned pigs by enhancing barrier integrity and inhibiting inflammation. The transcriptomics revealed that L. reuteri SXDT-32 protected against inflammatory injury by inhibiting the PI3K-AKT signaling pathway. Metabolite analysis indicated that the content of shikimic acid (SA) was substantially elevated in the colonic mucosa of L. reuteri SXDT-32-fed piglets (P < 0.05). In addition, Liquid Chromatography-Mass Spectrometer (LC-MS) analysis showed significant increases in SA content in both the colonic chyme of L. reuteri SXDT-32-fed piglets and the supernatant of in vitro grown cultures of L. reuteri SXDT-32 (P < 0.05). Polymerase chain reaction (PCR) analysis identified gene aroE from L. reuteri SXDT-32, which is a key gene directly linked to SA synthesis, and elevated shikimate dehydrogenase (SD, encoded by aroE) was also detected in both L. reuteri SXDT-32 and the colonic mucosa of piglets fed L. reuteri SXDT-32 (P < 0.01). In vitro Caco-2 cell experiments demonstrated that SA, L. reuteri SXDT-32, and the supernatant from in vitro grown cultures of L. reuteri SXDT-32 exhibited comparable inhibitory effects on the PI3K-Akt pathway to those of the PI3K inhibitor LY294002. Conclusions L. reuteri SXDT-32 alleviated intestinal inflammation in piglets by producing SA that inhibits the PI3K-Akt pathway. This study provides an innovative approach for the treatment and prevention of colitis caused by bacterial infection.