Characterization of a novel ETX/MTX2 domain-presenting parasporal crystal protein from Bacillus toyonensis HSY140 strain
文献类型： 外文期刊
作者： Jin, Yinghong 1 ; Huang, Yiming 1 ; He, Jiali 1 ; Wang, Yangyang 1 ; Xu, Yuquan 2 ; Wang, Ruiping 1 ; Zhang, Wenfei 1 ;
作者机构： 1.Hainan Normal Univ, Coll Life Sci, Minist Educ, Key Lab Trop Anim & Plant Ecol Hainan Prov,Key Lab, Haikou 571158, Peoples R China
2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
关键词： Bacillus toyonensis; parasporal crystal; genome; Mpp23-like protein; ETX/MTX2
期刊名称：SCIENCEASIA （ 影响因子：1.2； 五年影响因子：1.0 ）
ISSN： 1513-1874
年卷期： 2023 年 49 卷 5 期
页码：
收录情况： SCI
摘要： The soil-derived Bacillus toyonensis HSY140 strain was found to produce parasporal crystalline inclusions. Genome sequencing and annotation did not find the three-domain Cry (3d-Cry) and Cyt toxins, but a novel mpp23-like gene (918 bp) containing an ETX/MTX2 domain was detected. Alignment and phylogenetic analysis revealed that the translated Mpp23-like protein had the highest homology to Cry23Aa1 (now named Mpp23Aa1, AAA22333.1). Cloning and expression of the mpp23-like gene into the acrystalliferous Bacillus thuringiensis BMB171 (cry-) generated a recombinant strain able to produce elliptical parasporal crystals with the same shape as those of the HSY140 strain. Finally, bioassay experiments indicated that the expressed Mpp23-like protein did not exhibit any activity to the lepidopteran insects of Plutella xylostella, Helicoverpa armigera, and Spodoptera exigua and the dipteran insect of Aedes aegypti. Altogether, we have characterized for the first time a new toxin, Mpp23-like protein, responsible for the formation of crystalline inclusions in B. toyonensis HSY140 strain.
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