文献类型： 外文期刊

作者： Khan, Hamad ¹ ; Khan, Zeeshan ² ; Eman, Ishmal ² ; Ahmad, Ijaz ³ ; Shah, Tariq ¹ ; Wang, Guoping ¹ ; Feng, Lu ¹ ; Alarfaj, Abdullah A. ⁴ ; Alharbi, Sulaiman Ali ⁴ ; Ansari, Muhammad Javed ⁵ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Henan, Peoples R China

2.Natl Univ Sci & Technol, Atta ur Rehman Sch Appl Biosci, Dept Plant Biotechnol, Islamabad 44000, Pakistan

3.Univ Agr Peshawar, Fac Crop Prod Sci, Dept Plant Breeding, Peshawar 25130, Pakistan

4.King Saud Univ, Coll Sci, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia

5.Mahatma Jyotiba Phule Rohilkhand Univ Bareilly, Hindu Coll Moradabad, Dept Bot, Bareilly 244001, Uttar Pradesh, India

关键词： Antioxidant defense system; Arsenic contents; Chlorophyll contents; Growth traits; Putrescine

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：2.7； 五年影响因子：3.1 ）

ISSN： 0254-6299

年卷期： 2025 年 178 卷

页码：

收录情况： SCI

摘要： Arsenic (As) contamination in soil represents a major challenge to global agriculture, threatening crop productivity and food security, making the development of effective mitigation strategies essential for sustainable farming. Synthetic bacterial communities (SynCom) improve host plants ability to withstand As stress by several mechanisms. It is well known that polyamines (PAs) strengthen the antioxidant defence system, prevent ethylene formation, preserve cell pH, and shield plant cells from the damaging effects of As, and so forth; nevertheless, it is still unknown how SynCom modify PA metabolism to improve plant resistance to As. Pot experiment was carried out to evaluate how SynCom affects root PA homeostasis, hydrogen peroxide (metabolite associated with PA), genes encoding antioxidant system and expression and activities of PA- associated degrading and synthesizing enzymes in rice subjected to As. SynCom inoculated plants exhibited maximum growth attributes, gene expression of two plasma membrane intrinsic protein, leaf water potential, and chlorophyll contents than non-inoculated plants exposed to As stress. With increased activity of PA catabolic enzymes (copper-containing diamine oxidase, CuAO; polyamine oxidase, PAO) and putrescine synthases (ornithine decarboxylase; arginine decarboxylase, ADC), SynCom inoculated plants resulted in higher putrescine and cadaverine concentrations but lower spermidine and spermine contents. Under As stress, the SynCom inoculated plants resulted in up-regulation of spermine synthase gene, OsSPMS, and down-regulation of PA catabolic enzyme genes (OsCuAO6, OsCuAO8, OsCuAO1 and OsCuAO2) and PA synthase genes (OsADC2 and OsADC1). As stressed plants inoculated with SynCom had higher level of expression in OsPAO1, OsPAO2, OsPAO3 as compared to non-inoculated plants, stimulating reactive oxygen species-associated stress responsiveness signaling through low H2O2 levels by enhancing the genes encoding antioxidant defence system (OsCu/Zn-SOD, OsCAT1 and OsMn-SOD). The results of this study showed that SynCom can alter PA metabolism to improve plants' resistance to heavy metals like As. The inoculation of SynCom emerges as a promising strategy to enhance plant resilience against As toxicity by promoting positive interactions and regulatory stress-responsive pathways. Furthermore, the inoculation of SynCom is a viable approach capable of ameliorating heavy metal stress and improving the productivity of crops in the contaminated soil by fostering positive interactions and stress responsive regulatory mechanisms. (c) 2025 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.