Improvement of plant growth and fruit quality by introducing a phosphoribosylpyrophosphate synthetase mutation into Methylorubrum populi
文献类型： 外文期刊
作者： Zhang, Cong 1 ; Yao, Lu 1 ; Zhang, Ming-Ming 1 ; Tian, Dan-Dan 1 ; Wu, Jing 1 ; Hu, Yu-Zheng 1 ; Bao, Kai 5 ; Ma, Zeng-Xin 1 ; Tan, Ling-Ling 1 ; Yang, Song 1 ;
作者机构： 1.Qingdao Agr Univ, Sch Life Sci, 700 Changcheng Rd, Qingdao 266000, Peoples R China
2.Qingdao Agr Univ, Shandong Prov Key Lab Agr Appl Mycol, 700 Changcheng Rd, Qingdao 266109, Peoples R China
3.Qingdao Agr Univ, Qingdao Int Ctr Microbes Utilizing Biogas, 700 Changcheng Rd, Qingdao, Shandong, Peoples R China
4.Chinese Acad Agr Sci, Tobacco Res Inst, 11 Keyuanjingsi Rd, Qingdao, Shandong, Peoples R China
5.Hubei Univ, Sch Life Sci, 368 Youyi Ave, Wuhan 430062, Hubei, Peoples R China
6.Qingdao Agr Univ, Sch Marine Sci & Engn, 700 Changcheng Rd, Qingdao 266000, Peoples R China
7.Tianjin Univ, Minist Educ, Key Lab Syst Bioengn, 92 Weijin Rd, Tianjin 300072, Peoples R China
关键词： phyllosphere colonization; Methylorubrum populi; phosphoribosylpyrophosphate synthetase; cucumber; quality improvement
期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：3.2； 五年影响因子：3.8 ）
ISSN： 1364-5072
年卷期： 2025 年 136 卷 1 期
页码：
收录情况： SCI
摘要： Aims The aim of this study was to evaluate the impact of the introduction of a phosphoribosylpyrophosphate synthetase (PRS) mutation into a plant growth-promoting strain of Methylorubrum on the enhancement of phyllosphere colonization, with the ultimate goal of improving plant growth and quality.Methods and results A strain of Methylorubrum populi (named HS04) was isolated from the groundnut leaves and found to process the plant-promoting traits, including the ability to produce indole acetic acid, siderophore, 1-aminocyclopropane-1-carboxylate deaminase, and to fix nitrogen. The application via foliar spray significantly increased the fresh weight of cucumber seedlings cultivated in a standard growth chamber, with 43.0% higher than the control group. Genomic analysis revealed that the presence of an array of genes involved in plant growth promotion, including accD, aldB, and ltaE, as well as potential nitrogen-fixation-related genes, including nifA, bchlLNB, and bchXYZ, in the HS04 strain. The introduction of the PRS mutation (an aspartic acid to an asparagine residue, D38N) in the HS04 strain (named HS04PTR) enhanced the utilization capacity of low concentrations of methanol and multi-carbon sources (C2-C5 carbon sources). The HS04PTR strain indicated a notable enhancement in the phyllosphere colonization, with the subsequent application further promoting the growth of cucumber seedlings. An agricultural solar greenhouse experiment was thus performed to assess the efficiency of the HS04PTR strain, sprayed at low abundance, in improving the growth and quality of cucumber plants, including vitamin C, reducing sugars, and total sugars.Conclusions Our findings provide insights into the potential of Methylorubrum/Methylobacterium strains with the PRS mutations as an efficient inoculant for advantageous agricultural applications.
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