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UDP-N-acetylglucosamine pyrophosphorylase enhances rice survival at high temperature

文献类型： 外文期刊

作者： Xia, Saisai 1 ; Liu, He 1 ; Cui, Yuanjiang 1 ; Yu, Haiping 1 ; Rao, Yuchun 2 ; Yan, Yuping 1 ; Zeng, Dali 1 ; Hu, Jiang 1 ;

作者机构： 1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Peoples R China

2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China

关键词： DNA damage; heat stress; high-temperature adaptation; photosystem defects; reactive oxygen species; rice

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.152； 五年影响因子：10.476 ）

ISSN： 0028-646X

年卷期：

页码：

收录情况： SCI

摘要： High-temperature stress inhibits normal cellular processes and results in abnormal growth and development in plants. However, the mechanisms by which rice (Oryza sativa) copes with high temperature are not yet fully understood. In this study, we identified a rice high temperature enhanced lesion spots 1 (hes1) mutant, which displayed larger and more dense necrotic spots under high temperature conditions. HES1 encoded a UDP-N-acetylglucosamine pyrophosphorylase, which had UGPase enzymatic activity. RNA sequencing analysis showed that photosystem-related genes were differentially expressed in the hes1 mutant at different temperatures, indicating that HES1 plays essential roles in maintaining chloroplast function. HES1 expression was induced under high temperature conditions. Furthermore, loss-of-function of HES1 affected heat shock factor expression and its mutation exhibited greater vulnerability to high temperature. Several experiments revealed that higher accumulation of reactive oxygen species occurred in the hes1 mutant at high temperature. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and comet experiments indicated that the hes1 underwent more severe DNA damage at high temperature. The determination of chlorophyll content and chloroplast ultrastructure showed that more severe photosystem defects occurred in the hes1 mutant under high temperature conditions. This study reveals that HES1 plays a key role in adaptation to high-temperature stress in rice.

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