Establishment of a Triplex qPCR Assay for Differentiating Highly Virulent Genotype I Recombinant Virus From Low-Virulence Genotype I and Genotype II African Swine Fever Viruses Circulating in China
文献类型： 外文期刊
作者： Ding, Leilei 1 ; Ren, Tao 1 ; Bing, Guoxia 3 ; Wang, Zhigang 1 ; Wang, Baoyue 3 ; Ni, Jianqiang 3 ; Liu, Yuliang 3 ; Zhao, Rui 1 ; Zhu, Yuanmao 1 ; Li, Fang 1 ; Liu, Renqiang 1 ; Fu, Qiang 2 ; Tian, Zhijun 1 ; Bu, Zhigao 1 ; Sun, Encheng 1 ; Zhao, Dongming 1 ;
作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Natl African Swine Fever Para reference Lab,Natl H, Harbin 150069, Peoples R China
2.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Peoples R China
3.China Anim Dis Control Ctr, Beijing 100125, Peoples R China
4.Chinese Acad Agr Sci, Inst Western Agr, Changji 831100, Peoples R China
关键词： African swine fever virus; differential detection; genotype II; highly virulent genotype I; low virulent genotype I; triplex real-time quantitative PCR
期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：3.0； 五年影响因子：3.4 ）
ISSN： 1865-1674
年卷期： 2024 年 2024 卷
页码：
收录情况： SCI
摘要： African swine fever virus (ASFV) poses serious threats to the global swine industry, food safety, and the economy. Since August 2018, different types of ASFVs have successively emerged in China, making ASF diagnostics more challenging. The highly virulent genotype I recombinant virus has gradually become the prevalent dominant strain and is identified by sequencing several of its genes, which is time-consuming and expensive. Here, we developed a triplex real-time quantitative PCR (qPCR) assay based on the ASFV B646L, X64R, and MGF_360-14L genes to differentiate highly virulent genotype I recombinant viruses from low-virulence genotype I and genotype II viruses in China. This method has high sensitivity and a limit of detection of 10 copies/reaction for standard plasmids, as well as good specificity without cross-reactions with the viral nucleic acids of porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), pseudorabies virus (PRV), porcine circovirus 2 (PCV 2), porcine circovirus 3 (PCV 3), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), or porcine rotavirus (PoRV). Importantly, triplex qPCR can be used to quickly and accurately evaluate clinical samples and cell cultures infected with highly virulent genotype I virus, low-virulence genotype I virus, or genotype II virus. Thus, triplex qPCR provides an alternative tool for ASF surveillance in China.
- 相关文献
作者其他论文 更多>>
-
Integration of digital phenotyping, GWAS, and transcriptomic analysis revealed a key gene for bud size in tea plant (Camellia sinensis)
作者：Zhang, Shuran;Chen, Si;Fu, Zhilu;Li, Fang;Chen, Qiyu;Ma, Jianqiang;Chen, Liang;Chen, Jiedan;Chen, Yuanquan
关键词：
-
Transcriptomic and morphologic vascular aberrations underlying FCDIIb etiology
作者：Fang, Chuantao;Sun, Licheng;Lu, Yujia;Guo, Jingjing;Mei, Xinyu;Qi, Dashi;Fang, Chuantao;Zhang, Xiaodan;Yang, Lin;Wang, Min;Tan, Yanfeng;Zhang, Jinsen;Liu, Guoping;Zhang, Fayong;Zhao, Rui;Qi, Dashi;Fang, Chuantao;Zhang, Xiaodan;Yang, Lin;Wang, Min;Tan, Yanfeng;Zhang, Jinsen;Liu, Guoping;Zhang, Fayong;Zhao, Rui;Qi, Dashi;Fang, Chuantao;Zhang, Xiaodan;Yang, Lin;Wang, Min;Tan, Yanfeng;Zhang, Jinsen;Liu, Guoping;Zhang, Fayong;Zhao, Rui;Qi, Dashi;Fang, Chuantao;Liu, Yi;Xiao, Jianbo;Gao, Xin;Zhu, Li;Ren, Maozhi;Ma, Shaojie;Zhao, Rui;Zhao, Rui
关键词：
-
Soil compaction reduces the yield potential of densely planted maize (Zea mays L.) by disrupting root and shoot growth coordination
作者：Gao, Zhuohan;Wang, Zhigang;Gao, Zhuohan;Liang, Lu;Wang, Xinbing;Ding, Zaisong;Li, Congfeng;Liu, Zheng;Zhao, Ming;Zhou, Baoyuan;Zhen, Wenchao
关键词：Maize; High-density planting; Soil compaction; Grain yield; Root and shoot growth
-
Variation in the response of maize (Zea mays L.) root-shoot growth and grain yield to tillage practices under various soil compactions
作者：Gao, Zhuohan;Wang, Xinbing;Ding, Zaisong;Liang, Lu;Liu, Zheng;Li, Congfeng;Zhao, Ming;Zhou, Baoyuan;Gao, Zhuohan;Wang, Zhigang;Zhen, Wenchao
关键词：Maize; Tillage; Soil bulk density; Root growth; Shoot growth; Grain yield
-
Age-Related Water Use Pattern of the Introduced Species Atriplex canescens Was Influenced by Root Distribution in Desert
作者：Zhou, Hai;He, Zhibin;Liu, Bing;Ren, Heng;Yang, Qiyue;Kang, Jianjun;Wang, Shuai;Zhao, Wenzhi;Feng, Xiangyan;Li, Fang
关键词：
-
Physiological differences between Mo17 and B73 maize inbred lines under salt stress
作者：Zhang, Shujie;Wen, Xinyue;Zhou, Guangshun;Li, Fang;Han, Yanlai;Wang, Yi;Han, Yanlai;Wang, Yi;Wen, Xinyue;Cui, Pengjuan
关键词：Oxidative damage; plant growth; salt stress;
Zea mays L; ion balance -
Glycosyl structure guided design, synthesis and biological accessment of chitin degrading enzyme inhibitors with insecticidal activity
作者：Ai, Yin;Zhang, Yanjun;Li, Fang;Zhou, Xingyue;Dong, Yanhong;Zhang, Jianjun;Chen, Wei;Yang, Qing;Yang, Qing
关键词：Insecticide; C -glycoside; Inhibitor; Molecular dynamics simulations; Multitarget
