A Rapid Field-Visualization Detection Platform for Genetically Modified Soybean 'Zhonghuang 6106' Based on RPA-CRISPR
文献类型： 外文期刊
作者： Tao, Ran 1 ; Zhang, Jihong 1 ; Meng, Lixia 1 ; Gao, Jin 1 ; Miao, Chaohua 1 ; Liu, Weixiao 1 ; Jin, Wujun 1 ; Wan, Yusong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
2.Minist Agr & Rural Affairs, Inspect & Testing Ctr Ecol & Environm Risk Assessm, Beijing 100081, Peoples R China
关键词： CRISPR-Cas12a; recombinase polymerase amplification; GM soybean; nucleic acid detection; point-of-care testing
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2025 年 26 卷 1 期
页码：
收录情况： SCI
摘要： Genetically modified (GM) herbicide-tolerant soybean 'Zhonghuang 6106', which introduces a glyphosate-resistant gene, ensures soybean yield while allowing farmers to reduce the use of other herbicides, thereby reducing weed management costs. To protect consumer rights and facilitate government supervision, we have established a simple and rapid on-site nucleic acid detection method for GM soybean 'Zhonghuang 6106'. This method leverages the isothermal amplification characteristics of RPA technology and the high specificity of CRISPR-Cas12a to achieve high sensitivity and accuracy in detecting GM soybean components. By optimizing experimental conditions, the platform can quickly produce visual detection results, significantly reducing detection time and improving efficiency. The system can detect down to 10 copies/mu L of 'Zhonghuang 6106' DNA templates, and the entire detection process takes about 1 h. The technology also has strong editing capabilities; by redesigning the primers and crRNA in the method, it can become a specific detection method for other GM samples, providing strong technical support for the regulation and safety evaluation of GM crops.
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