文献类型： 外文期刊

作者： Liu, Kunyi ¹ ; Wang, Liyan ¹ ; Jiang, Bin ¹ ; An, Jiangshan ¹ ; Nian, Bo ¹ ; Wang, Daoping ⁸ ; Chen, Lijiao ¹ ; Ma, Yan ¹ ;

作者机构： 1.Yunnan Agr Univ, Coll Tea Sci, Kunming 650201, Yunnan, Peoples R China

2.Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Yunnan, Peoples R China

3.Yibin Vocat & Tech Coll, Coll Wuliangye Technol & Food Engn, Yibin 644003, Sichuan, Peoples R China

4.Yibin Vocat & Tech Coll, Coll Modern Agr, Yibin 644003, Sichuan, Peoples R China

5.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650201, Yunnan, Peoples R China

6.Yunnan Agr Univ, Key Lab Med Plant Biol Yunnan Prov, Kunming 650201, Yunnan, Peoples R China

7.Yunnan Agr Univ, Local Joint Engn Res Ctr Germplasm Innovat & Util, Kunming 650201, Yunnan, Peoples R China

8.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China

9.Tea Sci Res Inst Pu Erh City, Puer 665000, Yunnan, Peoples R China

关键词： Pu-erh tea; Penicillium chrysogenum; Enhanced fermentation; Chemical component; Fungal community; Sensory characteristic

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）

ISSN： 0963-9969

年卷期： 2021 年 150 卷

页码：

收录情况： SCI

摘要： Developing an effective method to improve the quality of Pu-erh tea is of great scientific and commercial interest. In this work, Penicillium chrysogenum P1 isolated from Pu-erh tea was inoculated in sterilized or unsterilized sundreid green tea leaves to develop pure-culture fermentation (PF) and enhanced fermentation (EF) of Pu-erh tea. Spectrophotometry and high performance liquid chromatography determined that contents of free amino acids (FAA), total tea polyphenols and eight polyphenolic compounds in PF were significantly lower than these in noninoculation control test (CK) (P < 0.05), whereas the contents of soluble sugars and theabrownins (TB) in PF were significantly higher (P < 0.05) than in CK. A total of 416 volatile compounds were detected by headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry. Comparison to CK, 124 compounds in PF were degraded or decreased significantly [Variable importance in projection [(VIP) > 1.0, P < 0.05, fold change (FC) < 0.5], whereas 110 compounds in PF were formed or increased significantly (VIP > 1.0, P < 0.05, FC > 2). Compared with normal fermentation (NF), the levels of gallic acid, (+)-catechin, (-)-epicatechin and 64 volatile compounds in EF were significantly lower (VIP > 1.0, P < 0.05, FC < 0.5), whereas the levels of FAA and 39 volatile compounds were significantly higher (VIP > 1.0, P < 0.05, FC > 2). Amplicon sequencing of fungal internal transcribed spacer 1 (ITS1) revealed that P. chrysogenum P1 didn't become the dominant fungus in EF; while the fungal communities in EF were different from those in NF, in that the relative abundances of Blastobotrys bambusae and P. chrysogenum in EF were higher, and the relative abundances of Aspergillus niger and Kluyveromyces marxianus in EF were lower. Overall, inoculation of P. chrysogenum in unsterilized sun-dreid green tea leaves changed the the fungal communities in fermentation of Pu-erh tea, and chemical compounds in fermented tea leaves, i.e., the levels of TB and the compounds responsible for the stale flavor, e.g., 2-amino-4-methoxybenzothiazole were increased, resulting in improvement of the sensory quality, including mellower taste and stronger stale flavor.