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Proteome-wide identification of methylglyoxalated proteins in rapeseed (Brassica napus L.)

文献类型： 外文期刊

作者： Fu, Zheng-Wei 1 ; Fan, Shi-Hang 1 ; Liu, Hong-Fang 1 ; Hua, Wei 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Oil Crops, Oil Crops Res Inst, Wuhan 430062, Peoples R China

关键词： Brassica napus; Abiotic stress; Methylglyoxal; Post-translational modification; Proteomics

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2024 年 207 卷

页码：

收录情况： SCI

摘要： Methylglyoxal (MG), a highly reactive cellular metabolite, is crucial for plant growth and environmental responses. MG may function by modifying its target proteins, but little is known about MG -modified proteins in plants. Here, MG -modified proteins were pulled down by an antibody against methylglyoxalated proteins and detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. We identified 543 candidate proteins which are involved in multiple enzymatic activities and metabolic processes. A great number of candidate proteins were predicted to localize to cytoplasm, chloroplast, and nucleus, consistent with the known subcellular compartmentalization of MG. By further analyzing the raw LC-MS/MS data, we obtained 42 methylglyoxalated peptides in 35 proteins and identified 10 methylglyoxalated lysine residues in a myrosinasebinding protein (BnaC06G0061400ZS). In addition, we demonstrated that MG modifies the glycolate oxidase and beta-glucosidase to enhance and inhibit the enzymatic activity, respectively. Together, our study contributes to the investigation of the MG -modified proteins and their potential roles in rapeseed.

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