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RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Ps gamma-COPI and PsArf1COPI as potential molecular targets for pest control

文献类型： 外文期刊

作者： Guo, Mujuan 1 ; Gao, Ran 1 ; Nanda, Satyabrata 2 ; Li, Yingqiu 1 ; Guo, Changfei 1 ; Zhou, Xuguo 3 ; Zhang, Youjun 4 ; Yang, Chunxiao 5 ; Pan, Huipeng 1 ;

作者机构： 1.South China Agr Univ, Coll Plant Protect, Natl Key Lab Green Pesticide, Guangzhou 510642, Peoples R China

2.Centurion Univ Technol & Management, MS Swaminathan Sch Agr, Paralakhemundi 761200, India

3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA

4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China

5.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou 510642, Peoples R China

关键词： Phyllotreta striolata; Reference gene; RNA interference; Feeding RNAi; Injection RNAi; Pest control

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2023 年 193 卷

页码：

收录情况： SCI

摘要： Phyllotreta striolata (Fabricius), commonly known as the striped flea beetle (SFB), is a notorious insect pest that attacks Brassicaceae plants worldwide, leading to tremendous economic losses. RNA interference (RNAi) has been proposed as a promising strategy for sustainable and eco-friendly pest control. In this study, a total of nine housekeeping genes including PsVATPA, PsHSP90, PsEF1A, PsRPL6, PsRPS24, PsActin, PsTUBA, PsRPS18, and PsRPL4 were evaluated under four different conditions (organization, population, sex, and RNAi). PsEF1A and PsVATPA were identified as the best reference genes for RNAi bioassay. Furthermore, a total of 24 target genes were selected to investigate their RNAi effects in SFB adults with double-stranded RNAs (dsRNAs), five of them showed significant mortality (28.00% to 70.00%), namely Ps alpha-COPI, Psi-COPI, PsRPS18, Ps gamma-COPI, and PsArf1COPI. We found that gene transcript levels of the two most lethal genes, Ps gamma-COPI and PsArf1COPI, were significantly decreased after treated with the target dsRNAs either by feeding or injection method. The findings from this study demonstrated that the introduction of dsRNAs via oral feedings or injection induces the RNAimediated silencing of target genes and can lead to insect mortality. Overall, the identified target genes can be explored in developing RNAi-based insecticides for SFB control.

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