文献类型： 外文期刊

作者： Zhang, Binglei ¹ ; Wang, Yihua ³ ; Zhu, Yun ¹ ; Pan, Tian ³ ; Yan, Haigang ³ ; Wang, Xin ¹ ; Jing, Ruonan ¹ ; Wu, Hongming ³ ; Wang, Fan ¹ ; Zhang, Yu ³ ; Bao, Xiuhao ³ ; Wang, Yongfei ³ ; Zhang, Pengcheng ³ ; Chen, Yu ¹ ; Duan, Erchao ³ ; Han, Xiaohang ¹ ; Wan, Gexing ¹ ; Yan, Mengyuan ¹ ; Sun, Xiejun ¹ ; Lei, Cailin ¹ ; Cheng, Zhijun ¹ ; Zhao, Zhichao ¹ ; Jiang, Ling ³ ; Bao, Yiqun ² ; Ren, Yulong ¹ ; Wan, Jianmin ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Natl Key Facil Crop Gene Resources & Genet Improve, Beijing 100081, Peoples R China

2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Peoples R China

4.Zhongshan Biol Breeding Lab, Nanjing 210095, Peoples R China

关键词： autophagic degradation; MON1-CCZ1; Rab7; rice; vacuolar trafficking

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.3； 五年影响因子：10.8 ）

ISSN： 1672-9072

年卷期： 2025 年 67 卷 1 期

页码：

收录情况： SCI

摘要： Autophagy is a highly conserved cellular program in eukaryotic cells which mediates the degradation of cytoplasmic components through the lysosome, also named the vacuole in plants. However, the molecular mechanisms underlying the fusion of autophagosomes with the vacuole remain unclear. Here, we report the functional characterization of a rice (Oryza sativa) mutant with defects in storage protein transport in endosperm cells and accumulation of numerous autophagosomes in root cells. Cytological and immunocytochemical experiments showed that this mutant exhibits a defect in the fusion between autophagosomes and vacuoles. The mutant harbors a loss-of-function mutation in the rice homolog of Arabidopsis thaliana MONENSIN SENSITIVITY1 (MON1). Biochemical and genetic evidence revealed a synergistic interaction between rice MON1 and AUTOPHAGY-RELATED 8a in maintaining normal growth and development. In addition, the rice mon1 mutant disrupted storage protein sorting to protein storage vacuoles. Furthermore, quantitative proteomics verified that the loss of MON1 function influenced diverse biological pathways including autophagy and vacuolar transport, thus decreasing the transport of autophagic and vacuolar cargoes to vacuoles. Together, our findings establish a molecular link between autophagy and vacuolar protein transport, and offer insights into the dual functions of the MON1-CCZ1 (CAFFEINE ZINC SENSITIVITY1) complex in plants.