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dctA, dctB, and dctD contribute to the utilization of C4-dicarboxylates, carbon, nitrogen, as well as virulence in Acidovorax citrulli

文献类型： 外文期刊

作者： Wang, Lan 1 ; Mijiti, Maihemuti 3 ; Abuduzhayier, Ayijiamali 3 ; Zhao, Tingchang 2 ; Guan, Wei 2 ; Yang, Lina 1 ; Yang, Yuwen 2 ;

作者机构： 1.Jilin Agr Univ, Coll Plant Protect, Changchun 130118, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

3.Xinjiang Agr Univ, Coll Agron, Key Lab Prevent & Control Invas Alien Species Agr, Coconstruct Minist & Prov,Minist Agr & Rural Affai, Urumqi 830052, Peoples R China

4.Chinese Acad Agr Sci, Natl Nanfan Res Inst Sanya, Sanya 572024, Peoples R China

关键词： Acidovorax citrulli; C4-dicarboxylate transport system; dctA; dctB; dctD

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 205 卷

页码：

收录情况： SCI

摘要： Bacterial fruit blotch (BFB), a destructive bacterial disease triggered by Acidovorax citrulli, affects cucurbit crops like watermelon and melon. The absorption and use of carbon sources are foundational for bacteria to successfully colonize host plants. C4-dicarboxylates are critical carbon and energy substances, and their transport is completed by the C4-dicarboxylate transport system (Dct) which plays an important role in typical bacterial metabolism. However, the role of dct genes have not been determined for A. citrulli. To clarify the biological roles of the Dct system-related genes in A. citrulli, we developed dctA1, dctA2, dctB, and dctD deletion mutants, as well as dctA1A2 double deletion mutant, with their corresponding complementary strains in the A. citrulli wild-type strain Aac5 in this study. The functions of Dct-related genes in A. citrulli were analyzed through phenotype assays, including pathogenicity, C4-dicarboxylates utilization, carbon and nitrogen utilization, biofilm formation, swimming motility, and qRT-PCR analysis. Compared to the wild-type strain, the pathogenicity, utilization of C4dicarboxylates, growth ability in vivo and in vitro, and seed adhesion ability of the mutant strains were significantly limited, while the biofilm formation ability was significantly improved. Additionally, the utilization of select carbon sources (glucose, maltose, and sucrose) and nitrogen sources ((NH4)2SO4, NH4Cl, CH4N2O, and KNO3) was significantly enhanced. qRT-PCR results demonstrated that the deletion of Dct-related genes resulted in significant downregulation of the expression of T3SS-related genes (hrpG and hrpE), the pili-related genes (pilA and pilN), and some flagellum-related genes (fliC, flhC, and flhD). These findings suggested that Dct-related genes were involved in C4-dicarboxylate utilization, carbon and nitrogen use, and the pathogenicity of A. citrulli.

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