Impacts of neonicotinoid compounds on the structure and function of Apis mellifera OBP14: Insights from SPR, ITC, multispectroscopy, and molecular modeling
文献类型： 外文期刊
作者： Li, Xiangshuai 1 ; Li, Shiyu 1 ; Zhao, Fangkui 1 ; Fu, Ruohan 2 ; Cui, Li 1 ; Chen, Shuning 1 ; Yang, Daibin 1 ; Yuan, Huizhu 1 ; Yan, Xiaojing 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China
关键词： Surface plasmon resonance; Multispectroscopy; OBP14; Isothermal titration calorimetry; Molecular dynamics
期刊名称：COLLOIDS AND SURFACES B-BIOINTERFACES （ 影响因子：5.6； 五年影响因子：5.4 ）
ISSN： 0927-7765
年卷期： 2025 年 250 卷
页码：
收录情况： SCI
摘要： Honeybees are vital for biodiversity and agricultural productivity, yet their populations are declining globally, partly due to exposure to neonicotinoid pesticides. Odorant-binding protein 14 (OBP14) plays an important role in honeybee chemosensation, but its involvement in neonicotinoid toxicity remains underexplored due to limitations in traditional fluorescence spectroscopy techniques. This gap hampers our understanding of neonicotinoid risks to honeybee health. Here, we explored the molecular interactions between OBP14 from Apis mellifera and three widely used neonicotinoids (imidacloprid, thiamethoxam, and clothianidin) using molecular modeling, surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), and multispectroscopy. SPR and ITC characterized the binding affinity, specificity, and thermodynamic parameters of AmelOBP14 interacting with three neonicotinoid compounds, revealing that the binding process is spontaneous and primarily driven by hydrophobic and electrostatic interactions. Molecular modeling highlighted that phenylalanine residue Phe54, near the binding site, plays a critical role in these interactions. UV-vis absorption spectroscopy and synchronous fluorescence spectroscopy (SFS) support slight changes in the microenvironment around the aromatic amino acids of OBP14. Fourier Transform Infrared Spectroscopy (FTIR) and circular dichroism spectroscopy (CD) indicate a decrease in the alpha-helix content of OBP14, suggesting a change in its secondary structure, while threedimensional (3D) fluorescence spectroscopy confirms the non-fluorescent nature of the OBP14 polypeptide backbone. The study results revealed its potential as a biomarker for pesticide risk assessment, providing important insights into the molecular mechanisms by which neonicotinoids may impair bee chemosensory function, and offering guidance for the design of safer pesticides to minimize harm to these important pollinators.
- 相关文献
作者其他论文 更多>>
-
Enhancing agrochemical delivery in citrus leaves with geraniol: a promising strategy for controlling huanglongbing (HLB)
作者：Chen, Shuning;Yuan, Ao;Wang, Huafeng;Yang, Daibin;Cui, Li;Hao, Fengjiao;Yan, Xiaojing;Yuan, Huizhu
关键词：citrus huanglongbing; chemical permeation; essential oils; adjuvants; fungicides; antimicrobials
-
Nanoscale Metal-Organic Frameworks for the Co-Delivery of Cycloxaprid and Pooled siRNAs to Enhance Control Efficacy in Diaphorina citri
作者：Wang, Wenjie;Ghafar, Muhammad Adeel;Liuyang, Lu;Haq, Inzamam Ul;Wang, Liande;Wang, Wenjie;Cui, Li;Yuan, Huizhu
关键词：agriculture; cycloxaprid;
Diaphorinacitri ; metal-organic frameworks; RNA pesticides -
The utilization of an ultrasonic mung bean protein-starch conjugate as a fat substitute in whipping cream
作者：Feng, Xuewei;Li, Shiyu;Jiang, Chunyang;Su, Hang;Yao, Yang;Feng, Xuewei;Zhu, Yingying;Zhang, Yongjiu;Hao, Xiyu;Hao, Xiyu
关键词：ultrasound; mung bean protein-starch conjugate; whipping cream; physicochemical properties; in vitro digestibility
-
Critical Role of Transcription Factor CtSR in Mediating Ergosterol Biosynthesis and DMI Fungicides Sensitivity in Colletotrichum truncatum
作者：Sun, Wei;He, Yongqi;Yuan, Huizhu;Lei, Tengyu;Yan, Xiaojing;Yang, Daibin;Chen, Shuning
关键词：ergosterol biosynthesis; DMI fungicides;
Colletotrichum spp. ; transcription factor; fungicides resistance -
Molecular mechanisms of cis-oxygen bridge neonicotinoids to Apis mellifera Linnaeus chemosensory protein: Surface plasmon resonance, multiple spectroscopy techniques, and molecular modeling
作者：Li, Xiangshuai;Li, Shiyu;Liu, Yang;Cui, Li;Yang, Daibin;Chen, Shuning;Yuan, Huizhu;Yan, Xiaojing;Shao, Xusheng
关键词：Chemosensory protein; Neonicotinoid; Surface plasmon resonance; Multispectroscopy; Molecular modeling
-
Invasion Dynamics and Migration Patterns of Fall Armyworm (Spodoptera frugiperda) in Shaanxi, China
作者：Yan, Zhanfeng;Wang, Xing;Yuan, Xiangqun;Li, Yiping;Yan, Zhanfeng;Feng, Xiaojun;Xie, Feizhou;Zhang, Yongjun;Yang, Daibin;He, Kanglai;Wang, Zhenying
关键词：invasive pest; migration route; molecular characterization; overwinter
-
Field Evaluation of Different Unmanned Aerial Spraying Systems Applied to Control Panonychus citri in Mountainous Citrus Orchards
作者：Cui, Zongyin;Han, Yifang;Yang, Weiguang;Zhan, Yilong;Wu, Jiapei;Qin, Yingdong;Chen, Pengchao;Lan, Yubin;Cui, Zongyin;Cui, Li;Yan, Xiaojing;Cui, Zongyin;Han, Yifang;Yang, Weiguang;Zhan, Yilong;Wu, Jiapei;Qin, Yingdong;Chen, Pengchao;Lan, Yubin;Chen, Pengchao;Lan, Yubin;Lan, Yubin
关键词：
Panonychus citri ; unmanned aerial spraying system; spray performance; control effect
