Multi-chromatic and multi-component lateral flow immunoassay for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified crops
文献类型： 外文期刊
作者： Yang, Yao 1 ; Zhang, Zini 1 ; Wang, Zhi 3 ; Pan, Ruxin 1 ; Wu, Huimin 1 ; Zhai, Shanshan 1 ; Wu, Gang 1 ; Fu, Wei 2 ; Gao, Hongfei 1 ;
作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Agr Genet Modified Organisms Traceabil, Minist Agr & Rural Affairs, Wuhan 430062, Peoples R China
2.Minist Agr & Rural Affairs, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China
3.Chinese Acad Inspection & Quarantine, Beijing 100176, Peoples R China
关键词： Multi-component analysis; Lateral flow immunoassay; Visual detection; Genetically modified crop; Polystyrene microspheres; On-site detection
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）
ISSN： 0026-3672
年卷期： 2025 年 192 卷 1 期
页码：
收录情况： SCI
摘要： A multi-chromatic and multi-component lateral flow immunoassay (MCMC-LFIA) was developed for simultaneous detection of CP4 EPSPS, Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins in genetically modified (GM) crops. Captured antibodies specific to these exogenous proteins were separately immobilized on a nitrocellulose membrane as test zones. Multi-colored microspheres, used as visible multi-probes, were conjugated with corresponding antibodies and sprayed on the conjugate pad. The assay results can be visually interpreted within 10 min by observing the appearance of colored bands. The MCMC-LFIA demonstrated high sensitivity, with detection of limits of 7.8 ng/mL for CP4 EPSPS and 2.5 ng/mL for Bt-Cry1Ab, Bt-Cry1Ac, and PAT/bar proteins, significantly improving the performance of previously reported LFIAs. The MCMC-LFIA exhibited excellent specificity and was validated for practical use in field-based applications. The proposed MCMC-LFIA offers a rapid, sensitive, and user-friendly tool for the on-site large-scale screening of GM materials.
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