Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection
文献类型： 外文期刊
作者： Gao, Shuji 1 ; Yuan, Shuo 1 ; Quan, Yingying 1 ; Jin, Wenjie 1 ; Shen, Yamin 1 ; Li, Rishun 1 ; Liu, Baobao 1 ; Wang, Yuxin 1 ; Yi, Li 3 ; Wang, Shaohui 1 ; Hou, Xiaogai 5 ; Wang, Yang 1 ;
作者机构： 1.Henan Univ Sci & Technol, Coll Anim Sci & Technol, Luoyang 471000, Peoples R China
2.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China
3.Henan Prov Engn Res Ctr Detect & Prevent & Control, Luoyang 471003, Peoples R China
4.Luoyang Normal Univ, Coll Life Sci, Luoyang 471934, Peoples R China
5.Henan Univ Sci & Technol, Coll Agr, Coll Tree Peony, Luoyang, Henan, Peoples R China
关键词：
期刊名称：VETERINARY RESEARCH （ 影响因子：3.5； 五年影响因子：4.0 ）
ISSN： 0928-4249
年卷期： 2025 年 56 卷 1 期
页码：
收录情况： SCI
摘要： The biofilm acts as a protective layer for Streptococcus suis (S. suis), contributing to the development of drug resistance and chronic infections. Autoinducer 2 (AI-2) quorum sensing represents the primary regulatory pathway governing biofilm formation in S. suis. Consequently, targeting AI-2 quorum sensing to inhibit biofilm formation represents a promising strategy for preventing and managing drug resistance and chronic infections caused by S. suis. This study established a small natural product library by integrating commercial drug molecules with Chinese herbal medicine molecules. Consequently, two natural products, salvianolic acid A (SAA) and rhapontin (RH), which target S. suis AI-2 via quorum sensing, were identified. SAA and RH inhibit AI-2 synthesis through noncompetitive and competitive binding to S-ribosylhomocysteinase (LuxS). By inhibiting S. suis AI-2 quorum sensing, these compounds modulate the expression of adhesion genes and the synthesis of extracellular polysaccharides (EPS), reducing the adhesion ability of S. suis and ultimately inhibiting biofilm formation. Using LC-MS/MS, we further analysed the impact of SAA and RH on the metabolic activity of S. suis, revealing the potential medicinal value of these compounds. Finally, the efficacy of SAA and RH against S. suis infection was validated in Galleria mellonella larvae, confirming their significant anti-infection effects.
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