N6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins
文献类型： 外文期刊
作者： Wang, Qian 1 ; Xu, Shuai 1 ; Shen, Wentao 1 ; Wei, Yanli 1 ; Han, Lu 1 ; Wang, Zhengxiang 1 ; Yu, Yingying 1 ; Liu, Minxuan 1 ; Liu, Junwen 1 ; Deng, Guohua 3 ; Chen, Hualan 3 ; Zhu, Qiyun 1 ;
作者机构： 1.Lanzhou Univ, Lanzhou Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Chinese Acad Agr Sci,Coll Vet Med, Lanzhou 730000, Peoples R China
2.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Peoples R China
3.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Harbin 150069, Peoples R China
关键词： influenza Avirus; N6-methyladenosine; viral RNA; viral ribonucleoprotein complex; replication
期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.1； 五年影响因子：10.6 ）
ISSN： 0027-8424
年卷期： 2025 年 122 卷 11 期
页码：
收录情况： SCI
摘要： N6-methyladnosine (m6A) modification is present in both positive-and negative-strand RNA of influenza A virus (IAV) and affects the replication and pathogenicity of IAV. However, little is known about the regulatory mechanism of m6A in IAV RNA. In the present study, we identified the m6A methylation of the viral RNA of different IAV subtypes and confirmed that m6A modification promotes the polymerase activity and replication of IAV. By mutating m6A motifs on the multiple viral RNAs (vRNAs) of IAV, we revealed that m6A deficiency in vRNA suppresses the expression of viral genes and the replication of the virus in vitro. In addition, m6A deficiency in vRNA reduced the pathogenicity of IAV in a mouse model. Mechanistically, m6A deficiency in vRNA suppresses the assembly of the viral ribonucleoprotein (vRNP) complex by impairing the interaction between vRNA and vRNP proteins in an m6A methyltransferase-dependent manner, but not the m6A reader proteins. Together, our findings reveal an important role for m6A on viral RNAs in facilitating the activity of the polymerase complex and the replication and pathogenicity of IAV, which provides insights for the development of novel anti-influenza strategies.
- 相关文献
作者其他论文 更多>>
-
Near complete genome assembly of Yadong trout (Salmo trutta)
作者：Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Li, Chen;Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Shao, Changwei
关键词：
-
Shaker K+ channel NKT3A enhances potassium uptake and transport in tobacco (Nicotiana tabacum L.) seedlings under low potassium stress
作者：Xiang, Haiying;Xu, Li;Zhang, Jianduo;Mi, Qili;Gao, Qian;Yang, Wenwu;Huang, Haitao;Wang, Kunmiao;Zeng, Wanli;Yuan, Guang;Shi, Chuhan;Ning, Yang;Wang, Qian;Yuan, Guang;Shi, Chuhan
关键词：Tobacco; Potassium; Shaker K+ channel NKT3A; Uptake and transport
-
Genetic dissection of a major locus SC9.1 conferring seed color in broomcorn millet (Panicum miliaceum)
作者：Liu, Tianpeng;Dong, Kongjun;He, Jihong;Ren, Ruiyu;Zhang, Lei;Li, Yawei;Yang, Tianyu;Liu, Tianpeng;Dong, Kongjun;He, Jihong;Ren, Ruiyu;Zhang, Lei;Li, Yawei;Yang, Tianyu;Wang, Mei;Liu, Minxuan
关键词：Broomcorn millet; Seed color; BSA-seq; Fine mapping; Transcriptome; Haplotype
-
The full-length nsp2 replicase contributes to viral assembly in highly pathogenic PRRSV-2
作者：Bai, Yuan-Zhe;Wang, Shujie;Sun, Yue;Liu, Yong-Gang;Zhang, Hong-Liang;Wang, Qian;Huang, Rui;Rao, Cui-Hong;Xu, Shi-Jia;Tian, Zhi-Jun;An, Tong-Qing;Cai, Xue-Hui;Tang, Yan-Dong;Cai, Xue-Hui;Tang, Yan-Dong;Tang, Yan-Dong
关键词：arterivirus; PRRSV; nsp2; assembly; glycoprotein (GP); nucleocapsid (N) protein
-
Cnidii fructus and Sophorae Flavescentis Radix polysaccharides inhibit SARS-CoV-2 entry by interfering with Spike protein-mediated membrane fusion
作者：Liu, Feng;Jiang, Xuyong;Lin, Yanling;Wu, Fushan;Li, Jingxuan;Han, Lu;Shan, Junjie;Xiao, Zhiyong;Zhou, Wenxia;Liu, Feng;Jiang, Xuyong;Lin, Yanling;Wu, Fushan;Li, Jingxuan;Han, Lu;Shan, Junjie;Xiao, Zhiyong;Zhou, Wenxia;Shang, Chao;Zhang, Cheng
关键词：Cnidii fructus; Sophorae Flavescentis Radix; Polysaccharides; SARS-CoV-2; Fusion
-
Phenological Stage and Nitrogen Input Coordinately Regulate Bud Bank Dynamics and Shoot Allocation in an Alpine Clonal Perennial Grass
作者：He, Keyan;He, Lin;He, Lili;Dang, Haihong;Wei, Xiaoxing;He, Keyan;He, Lin;He, Lili;Dang, Haihong;Wei, Xiaoxing;Zhou, Qingping;Wang, Qian;Wang, Jiahao
关键词：belowground bud bank; nitrogen addition; phenological stage; shoot allocation; Qinghai-Tibet Plateau
-
Effects of Double-Stranded RNA Degrading Nucleases on RNAi Efficiency in Beet Moth Spodoptera exigua (Lepidoptera: Noctuidae)
作者：Wang, Guandi;Wang, Qian;Liu, Wenrui;Wen, Jingxin;Yang, Yubo;Niu, Zhilong;Guo, Wei;Zhao, Dan;Guo, Wei
关键词：RNA interference; dsRNA stability; dsRNase;
Spodoptera exigua ; nanomaterial-mediated RNAi
