文献类型： 外文期刊

作者： Liu, Qingqing ¹ ; Sun, Zhipeng ² ; Liu, Yufang ¹ ; He, Xiaoyun ¹ ; Ren, Chunhuan ² ; Wang, Xiangyu ¹ ; Di, Ran ¹ ; Zhao, Yongju ³ ; Zhang, Zijun ² ; Chu, Mingxing ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Anim Sci, State Key Lab Anim Biotech Breeding, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China

2.Anhui Agr Univ, Coll Anim Sci & Technol, 130 Changjiang West Rd, Hefei 230036, Peoples R China

3.Southwest Univ, Coll Anim Sci & Technol, Chongqing Key Lab Herbivore Sci, Chongqing 400715, Peoples R China

关键词： Goat; Oviduct; microRNA; ceRNA regulatory network; Reproduction trait

期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）

ISSN： 1471-2164

年卷期： 2025 年 26 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundReproduction traits are crucial for livestock breeding and represent key economic indicators in the domestic goat (Capra hircus) industry. The oviduct, a critical organ in female mammals, plays a pivotal role in several reproductive processes; however, its molecular mechanisms remain largely unknown. Non-coding RNA and mRNAs are essential regulatory elements in reproductive processes; yet their specific roles and regulatory networks in goat oviducts remain unclear.ResultsIn this study, we conducted small RNA sequencing of the oviduct in high- and low-fecundity goats during the follicular (FH and FL groups, n = 10) and luteal (LH and LL groups, n = 10) phase, profiling 20 tissue samples. Combinatorial whole-transcriptome expression profiles were applied to the same samples to uncover the competitive endogenous RNA (ceRNA) regulation network associated with goat fecundity. RT-qPCR was employed to validate the miRNA profiling results, and ceRNA regulatory networks were analyzed through luciferase assay. Gene set enrichment analysis (GSEA) confirmed that the cytokine-cytokine receptor interaction and TGF-beta signaling pathway, both related to embryonic development, were enriched in DEM target genes. Additionally, miR-328-3p, a core miRNA, targets SMAD3 and BOP1, which are involved in the negative regulation of cell growth and embryonic development. TOB1 and TOB2, targeted by miR-204-3p, regulate cell proliferation via the protein kinase C-activating G-protein coupled receptor signaling pathway. Analyses of ceRNA regulatory networks revealed that LNC_005981 - miR-328-3p - SMAD3 and circ_0021923 - miR-204-3p - DOT1L may affect goats' reproduction, findings that were validated using luciferase assay.ConclusionAnalysis of whole-transcriptome profiling of goat oviducts identified several key miRNAs and ceRNAs that may regulate oocyte maturation, embryo development, and the interactions between the oviduct and gametes/early embryos, providing insights into the molecular mechanisms of reproductive regulatory networks.