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CRISPR/Cas9-mediated knockout of NtMYC2a gene involved in resistance to bacterial wilt in tobacco

文献类型： 外文期刊

作者： Xiao, Zhiliang 2 ; Yang, Wenwu 1 ; Yang, Aiguo 2 ; Deng, Lele 1 ; Geng, Ruimei 2 ; Xiang, Haiying 1 ; Kong, Weisong 1 ; Jiang, Caihong 2 ; Li, Xuemei 1 ; Chen, Zhiqiang 2 ; Gao, Qian 1 ;

作者机构： 1.Yunnan Acad Tobacco Sci, Kunming 650106, Peoples R China

2.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao, Peoples R China

关键词： CRISPR/Cas9; MYC2; Transcriptome; Tobacco Bacterial wilt

期刊名称：GENE （ 影响因子：2.6； 五年影响因子：2.7 ）

ISSN： 0378-1119

年卷期： 2024 年 927 卷

页码：

收录情况： SCI

摘要： MYC2 is a class of bHLH family transcription factors and a major regulatory factor in the JA signaling pathway, and its molecular function in tobacco has not been reported. In this study, CRISPR/Cas9-mediated MYC2 gene NtMYC2a knockout mutants at tobacco was obtained and its agronomic traits, disease resistance, and chemical composition were identified. Comparing with the WT, the leaf width of the KO-NtMYC2a was narrowed, the nornicotine content and mecamylamine content increased significantly and the resistance to Ralstonia solanacearum significantly decreased. The transcriptome sequencing results showed that DEGs related to immunity, signal transduction and growth and development were enriched between KO-NtMYC2a and WT. NtJAR1 and NtCOI1 in KO-NtMYC2a were down-regulated to regulating the JA signaling pathway, result in a significant decrease in tobacco's resistance to R. solanacearum. Our research provides theoretical support for the functional research of MYC2 and the study of the mechanism of tobacco bacterial wilt resistance.

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