PHB2 binds to ER13 to induce the autophagy of bovine ovarian granulosa cells through mTOR phosphorylation
文献类型： 外文期刊
作者： Liu, Guangyu 1 ; Wang, Yifan 1 ; Zheng, Yuxin 1 ; Lv, Jing 1 ; Li, Yuanyou 1 ; Liu, Ning 1 ; Gao, Huimin 1 ; Ran, Haohan 2 ; Tang, Hong 3 ; Jiang, Zhongliang 1 ;
作者机构： 1.Northwestern A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China
2.Tianjin Agr Univ, Coll Anim Sci & Anim Med, Tianjin, Peoples R China
3.Xinjiang Acad Agr & Reclamat Sci, Inst Anim Husb & Vet Med, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Peoples R China
关键词： Prohibitin 2; ER13; Bovine; Ovary; Autophagy
期刊名称：THERIOGENOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）
ISSN： 0093-691X
年卷期： 2023 年 198 卷
页码：
收录情况： SCI
摘要： Autophagy of ovarian granulosa cells is one of the reasons which results in follicular atresia. PHB2 regulates many fundamental biological processes and is pivotal in the mitophagy of cells; nevertheless, the autophagy in the bovine ovary and how PHB2 regulates the follicular cells are unknown. Here we report a protein complex that induces autophagy in bovine granulosa cells (BGCs) through the direct interaction of ER13 and PHB2. In this study, we aimed to elucidate the autophagy and the role of PHB2 in bovine ovaries using bovine primary ovarian granulosa cells (BGCs). The results showed that PHB2 in-duces BGCs autophagy because of the change in related genes and protein expression levels. In addition, the results of Co-IP and the distribution of the combination of PHB2 and ER13 showed that this complex is also indicated as an essential role of PHB2 in BGCs autophagy. Based on our results, it can be concluded that PHB2 combined with ER13 induces BGCs autophagy by targeting the mTOR pathway. This study pinpoints a novel regulatory mechanism of autophagy and demonstrates the existence of a protein complex that may underlie its roles in autophagy in BGCs.(c) 2022 Published by Elsevier Inc.
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