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Characterizations of protein extracted from Moso bamboo shoot

文献类型： 外文期刊

作者： Pan, Weichun 1 ; Li, Zhe 1 ; Tu, Weiwei 1 ; Hu, Xinyu 1 ; Yang, Jinlai 2 ; Gao, Ying 3 ; Yang, Huimin 2 ; Ritzoulisa, Christos 1 ; Niu, Fuge 1 ; Wu, Liangru 2 ;

作者机构： 1.Zhejiang Gongshang Univ, Sch Food Sci & Biotechnol, Hangzhou 310018, Peoples R China

2.China Natl Bamboo Res Ctr, Hangzhou 310012, Zhejiang, Peoples R China

3.Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China

4.Amer Farm Sch, Thessaloniki 57001, Greece

5.Key Lab State Forestry & Grassland Adm Bamboo Fore, Hangzhou 310012, Zhejiang, Peoples R China

关键词： Moso bamboo shoot; Nanoparticles; Physicochemical properties

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 304 卷

页码：

收录情况： SCI

摘要： Low economic returns limit the widespread cultivation of bamboo, which could otherwise offer solutions to several global needs. This study aims to explore the physicochemical properties of nanoparticles (NPs) in protein solutions extracted from Moso bamboo shoots to enhance bamboo shoot applications. A 10 kDa molecular weight cutoff filter is used for protein purification, while amino acid composition analysis, SDS-PAGE, three independent spectroscopic techniques, and light scattering are employed for characterization. The purified protein was found to consist of 18 amino acids with molecular weights spreading from 30 kDa to 50 kDa. The corresponding secondary structure was also evaluated: Coiled, poly-dispersed NPs exhibited negative surface charges with sizes of ca 100 nm. rho-Ratio decreases upon dilution operation, indicating the NPs' topology variation. Stability studies reveal that the NPs were temperature-sensitive, and electrostatic interactions between protein molecules play a key role in regulating the size and shape of the NPs.

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