Protein phosphorylation networks in Baylisascaris procyonis revealed by phosphoproteomic analysis
文献类型： 外文期刊
作者： Meng, Qin 1 ; Li, Zhikang 1 ; Qiu, Qiguan 2 ; Chen, Shuyu 3 ; Gong, Haiyan 4 ; Tan, Xiaoruo 1 ; Liu, Xiaoheng 1 ; Chen, Zhaoguo 4 ; Liu, Wei 1 ;
作者机构： 1.Hunan Agr Univ, Coll Vet Med, Res Ctr Parasites & Vectors, Changsha 410128, Hunan, Peoples R China
2.Changsha Ecol Zoo, Changsha 410119, Hunan, Peoples R China
3.Anim Husb & Fisheries Affairs Ctr Huaihua, Huaihua 418000, Peoples R China
4.Chinese Acad Agr Sci, Key Lab Anim Parasitol, Lab Qual & Safety Risk Assessment Anim Prod Biohaz, Minist Agr,Shanghai Vet Res Inst,Minist Agr, Shanghai 200241, Peoples R China
关键词：
期刊名称：PARASITES & VECTORS （ 影响因子：3.5； 五年影响因子：3.5 ）
ISSN： 1756-3305
年卷期： 2025 年 18 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundBaylisascaris procyonis is an intestinal ascarid worm that parasitizes in raccoons and causes fatal neural, visceral, and ocular larva migrans in humans. Phosphorylated proteins and protein kinases have been studied as vaccine and drug target candidates against parasitic infections. However, no data are available on protein phosphorylation in the raccoon roundworm.MethodsIn this study, the entire proteome of adult B. procyonis was enzymatically digested. Then, phosphopeptides were enriched using immobilized metal affinity chromatography (IMAC) and analyzed by liquid chromatography-mass spectrometry (LC-MS/MS).ResultsOur phosphoproteome analysis displayed 854 unique phosphorylation sites mapped to 450 proteins in B. procyonis (3308 phosphopeptides total). The annotated phosphoproteins were associated with various biological processes, including cytoskeletal remodeling, supramolecular complex assembly, and developmental regulation. The phosphopeptide functional enrichment revealed that B. procyonis phosphoproteins were mostly involved in the cytoskeleton cellular compartment, protein binding molecular function, and multiple biological processes, including regulating supramolecular fiber and cytoskeleton organization and assembling cellular protein-containing complexes and organelles. The significantly enriched pathways of phosphoproteins included the insulin signaling pathway, tight junction, endocytosis, longevity-regulating, glycolysis/gluconeogenesis, and apelin signaling pathways. Domain analysis revealed that the Src homology 3 domain was significantly enriched.ConclusionsThis study presents the first phosphoproteomic landscape of B. procyonis, elucidating phosphorylation-mediated regulation of cytoskeletal dynamics, host interaction pathways, and metabolic adaptations. The identified 450 phosphoproteins and enriched functional domains establish a foundation for targeting conserved mechanisms critical to B. procyonis survival.
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