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Pigeon novel-miR-741 targets OTUD1 to inhibit proliferation and promote apoptosis of crop fibroblasts

文献类型： 外文期刊

作者： Zhang, Ran 1 ; Li, Yunlei 1 ; Li, Xinyi 1 ; Ni, Aixin 1 ; Gao, Yuxin 2 ; Zong, Yunhe 1 ; Sun, Yanyan 1 ; Yuan, Jingwei 1 ; Chen, Jilan 1 ; Ma, Hui 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Inst Anim Sci, Key Lab Anim Poultry Genet Breeding & Reprod, Beijing 100193, Peoples R China

2.Shanxi Agr Univ, Coll Anim Sci, Taigu 030800, Peoples R China

关键词： pigeon; Trichomonas gallinae; crop fibroblast; novel-miR-741; OTUD1

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 5 期

页码：

收录情况： SCI

摘要： Trichomonas gallinae (T. gallinae ) is a globally distributed protozoan parasite and could cause serious damage to the pigeon industry. MiRNAs have important roles in regulating parasite infection, but its impacts on T. gallinae resistance have rarely been reported. In the present study, we identified a new miRNA (novel-miR-741) and its predicted target OTU deubiquitinase 1 ( OTUD1 ) that might be associated with immunity to T. gallinae in pigeon. Novel-miR-741 and OTUD1 over -expression vectors and interference vectors were constructed. Results from dual luciferase activity assay demonstrated that OTUD1 was a downstream target of novel-miR-741. The Cell Counting Kit8 and apoptosis assays showed that novel-miR-741 inhibited the proliferation and promoted apoptosis of pigeon crop fibroblasts. Meanwhile, mRNA levels of OTUD1 were significantly reduced in novel-miR-741 mimic-transfected fibroblasts, while mRNA levels of OTUD1 were significantly increased in the novel-miR741 inhibitor-transfected fibroblasts. The regulatory roles of si- OTUD1 on fibroblasts proliferation, apoptosis, and migration were similar to novel-miR-741 mimic. Our findings demonstrated that novel-miR-741 inhibited the proliferation, and migration of crop fibroblasts, while OTUD1 promoted the proliferation and migration of crop fibroblasts. Therefore, the regulation of OTUD1 by novel-miR-741 was proposed as a potential therapeutic strategy for T. gallinae .

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