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Isolation, cloning, and characterization of a novel GH5 cellulase from yak rumen metagenome for enhanced lignocellulose hydrolysis in biofuel production and ruminant feed utilization

文献类型： 外文期刊

作者： Bature, Ibrahim 1 ; Liang, Zeyi 1 ; Xiaohu, Wu 1 ; Yang, Feng 1 ; Yang, Yayuan 1 ; Dong, Pengcheng 1 ; Ding, Xuezhi 1 ;

作者机构： 1.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Yak Breeding Engn, Lanzhou, Gansu, Peoples R China

2.Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab Vet Pharmaceut Dev, Minist Agr & Rural Affairs, Lanzhou, Gansu, Peoples R China

3.Fed Univ Dutsin Ma, Dept Anim Sci, Dutsin Ma, Katsina State, Nigeria

4.State Key Lab Herbage Improvement & Grassland Agro, Lanzhou, Peoples R China

5.Minist Agr & Rural Affairs, Key Lab Grassland Livestock Ind Innovat, Lanzhou, Peoples R China

6.Minist Educ, Engn Res Ctr Grassland Ind, Lanzhou, Peoples R China

7.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou, Peoples R China

关键词： Lignocellulosic biomass; Biofuel production; Enzymatic degradation; Enzyme stability; Glycoside hydrolase family 5

期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.4 ）

ISSN： 0141-0229

年卷期： 2025 年 191 卷

页码：

收录情况： SCI

摘要： Lignocellulosic biomass is a promising feedstock for biofuel production, but its complex structure, including cellulose and hemicellulose, challenges efficient enzymatic hydrolysis. CelyA, a novel cellulase from the yak rumen, has potential for improving biomass degradation and biofuel production. The CelyA gene was cloned, expressed, and purified. Biochemical characterization included assessments of pH, temperature, and salt tolerance. The enzyme's substrate specificity was tested on crystalline cellulose, CMC, and other polysaccharides. In vitro rumen fermentation was performed to evaluate its effect on fiber digestibility and microbial community composition. Biofuel production was tested by hydrolyzing maize, rice, and wheat straw. CelyA demonstrated optimal activity at pH 6.0 and 40.0 degrees C and maintained moderate stability across a wide pH range (3.0-12.0), retaining measurable activity even under strongly acidic and alkaline conditions. The enzyme demonstrated excellent salt tolerance, retaining 91.0 % activity in 1.0 M NaCl. CelyA efficiently degraded maize straw in hydrolysis assays, producing 7.2 mu mol/L of reducing sugars. In vitro rumen fermentation with CelyA increased fiber digestibility by 8.3 % for maize straw, 14.5 % for rice straw, and 2.7 % for wheat straw. Gas production also increased significantly, with maize straw showing a 91.3 % increase. 16S rRNA sequencing revealed selective enrichment of Ruminococcus and Prevotella, key cellulolytic microbes. CelyA demonstrates strong potential for biofuel production, efficiently hydrolyzing lignocellulosic biomass and enhancing ruminal fiber digestibility. Its stability, salt tolerance, and substrate specificity make it a valuable enzyme for biofuel production and livestock feed optimization.

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