文献类型： 外文期刊

作者： Ali, Murad ¹ ; Shaukat, Fiza ³ ; Khan, Waqar ⁴ ; Syed, Ayesha ⁵ ; Maqsood, Javaria ⁶ ; Kamal, Hira ⁷ ; Ercisli, Sezai ⁸ ; Balijagic, Jasmina ¹⁰ ; Ullah, Riaz ¹¹ ; Bari, Ahmed ¹² ; Anwar, Zunaira ¹³ ; Ijaz, Aqsa ¹³ ; Kashif, Muhammad ¹⁴ ; Khalid, Muhammad Nouman ¹⁴ ; Mustafa, Hafiz Saad Bin ¹⁵ ; Zafar, Muhammad Mubashar ¹⁶ ;

作者机构： 1.Hazara Univ, Dept Bot, Mansehra, Pakistan

2.Chinese Acad Agr Sci, Inst Crop Sci, Grad Sch, Beijing, Peoples R China

3.Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol, Faisalabad, Pakistan

4.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

5.Univ Agr Faisalabad, Natl Inst Food Sci & Technol, Faisalabad, Pakistan

6.Govt Coll Women Univ Sialkot, Dept Bot, Sialkot, Pakistan

7.Washington State Univ, Dept Plant Pathol, Pullman, WA USA

8.Ataturk Univ, Fac Agr, Dept Hort, TR-25240 Erzurum, Turkiye

9.Ata Teknokent, HGF Agro, TR-25240 Erzurum, Turkiye

10.Univ Montenegro, Biotech Fac, Podgorica 81000, Montenegro

11.King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh, Saudi Arabia

12.King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, Riyadh, Saudi Arabia

13.Pakistan Inst Engn & Appl Sci PIEAS, Nucl Inst Agr & Biol Coll NIAB C, Islamabad 45650, Pakistan

14.Univ Agr Faisalabad, Dept Plant Breeding & Genet, Faisalabad, Pakistan

15.Ayub Agr Res Inst Faisalabad, Directorate Oilseeds, Faisalabad, Pakistan

16.Chinese Acad Agr Sci, Inst Cotton Res, Anyang, Peoples R China

关键词： Core collection; SSR markers; Population structure; Genetic diversity

期刊名称：CELLULAR AND MOLECULAR BIOLOGY （ 影响因子：1.6； 五年影响因子：1.6 ）

ISSN： 0145-5680

年卷期： 2023 年 69 卷 10 期

页码：

收录情况： SCI

摘要： Illustrating the population structure and genetic diversity in selected germplasm resources (after three-year multi locations trials) plays a key role that directly utilizes the selection of lines in a population for accumulative trait breeding in crops. In order to further understand, the structure of population and genetic variability, this study explored 100 selected lines, conducted for three consecutive years (2016-2019) in swat, University of Malakand, Khyber Pakhtunkhwa Pakistan and Provinces of China (Chongqing and Beijing) with 33 mapped SSR markers. Through an integrated population structure analysis, the study examined a core set of one hundred germplasm of Pakistani origin, along with three approved commercial barley cultivars. The analysis revealed a robust stratification within the population, resulting in the classification of the germplasm into four major subpopulations (PI, PII, PIII, and PIV), as well as an admixture subpopulation consisting of 52, 9, 15, and 27 germplasm, respectively. A total of 133 alleles were identified with a mean value of 0.80 Polymorphic information content. The number of alleles detected by the system varied from two alleles amplified to six with an average of 4.03 per SSR marker pair. The gene diversity ranged from 0.56 to 0.98 with an average of 0.82 in selected germplasm resources. Based on the SSR data, the 100 selected germplasm with three cultivars were classified into four main phylogenetic lineages (LI, LII, LIII and LIV) which corresponded to the phylogenic grouping in genotypes. We assembled a core set of 20 barley genotypes (similar to 1/5 of original population size) to sustain sufficient mapping of SSR marker with Phenotype, in which we proposed four SSR markers, Bmac0040, Bmac0134, Bmag0125 and Bmag0211 for malt gene and marker (Bmac0399) for tolerance to salinity gene, which will be applicable for marker-assisted breeding in barley gene resources. Copyright: (c) 2023 by the C.M.B. Association. All rights reserved.