文献类型： 外文期刊

作者： Li, Ke ¹ ; Wang, Juan ¹ ; Liu, Chuanliang ¹ ; Li, Changsheng ¹ ; Qiu, Jingjing ¹ ; Zhao, Chuanzhi ¹ ; Xia, Han ¹ ; Ma, Changle ¹ ; Wang, Xingjun ¹ ; Li, Pengcheng ¹ ;

作者机构： 1.1College of Life Sciences, Shandong University, Qingdao 266237, People’s Republic of China

2.College of Life Sciences, Shandong Normal University, Jinan 250014, People’s Republic of China

3.4Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, People’s Republic of China

4.Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, People’s Republic of China

5.College of Life Sciences, Shandong University, Qingdao 266237, People’s Republic of China;Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, People’s Republic of China;College of Life Sciences, Shandong Normal University, Jinan 250014, People’s Republic of China

6.Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, People’s Republic of China;College of Life Sciences, Shandong Normal University, Jinan 250014, People’s Republic of China

关键词： GR; Dexamethasone; AtLEC2; AtIPT7; AtIPT9; Embryogenic callus; Shoot regeneration; Transcriptome analysis

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2019 年 19 卷

页码：

收录情况： SCI

摘要： BackgroundLEAFY COTYLEDON 2 (LEC2) acts throughout embryo morphogenesis and maturation phase to maintain embryogenic identity. Our previous study stated that Arabidopsis thaliana LEC2 (AtLEC2) driven by glucocorticoid receptor-dexamethasone (GR-DEX) inducible system (AtLEC2-GR) triggers embryogenic callus formation in tobacco (Nicotiana tabacum).ResultsIn this study, the adenosine phosphate isopentenyltransferase genes AtIPT3, AtIPT7 and the tRNA isopentenyltransferase gene AtIPT9 were overexpressed in the AtLEC2-GR transgenic background. In the AtIPT7-OE AtLEC2-GR and AtIPT9-OE AtLEC2-GR seedlings, high-quality embryogenic callus was obtained under the DEX condition, and the shoot regeneration efficiency was 2 to 3.5 folds higher than AtLEC2-GR alone on hormone free medium without DEX. Transcriptome analyses showed that up-regulated BBM, L1L, ABI3, and FUS3 might function during embryogenic callus formation. However, at the shoot regeneration stage, BBM, L1L, ABI3, and FUS3 were down-regulated and Type-B ARRs were up-regulated, which might contribute to the increased shoot regeneration rate.ConclusionsA novel system for inducing shoot regeneration in tobacco has been developed using the GR-DEX system. Induced expression of AtLEC2 triggers embryogenic callus formation and overexpression of AtIPT7 or AtIPT9 improves shoot regeneration without exogenous cytokinin.