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GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress

文献类型： 外文期刊

作者： Li, Zhi 1 ; Li, Lei 1 ; Zhou, Kehai 1 ; Zhang, Yihao 1 ; Han, Xiao 1 ; Din, Yanpeng 1 ; Ge, Xiaoyang 1 ; Qin, Wenqiang 1 ; Wang, Peng 1 ; Li, Fuguang 1 ; Ma, Zhiying 1 ; Yang, Zhaoen 1 ;

作者机构： 1.State Key Laboratory of Cotton Biology (Hebei Base), College of Agronomy, Hebei Agricultural University, Baoding, China;Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China

2.Anyang Hospital of Traditional Chinese Medicine, Anyang, China

3.State Key Laboratory of Cotton Biology (Hebei Base), College of Agronomy, Hebei Agricultural University, Baoding, China

关键词： Gossypium hirsutum; ABA signaling; drought; salt; negative regulation

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN： 1664-8021

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Drought and high salinity are key limiting factors for cotton production. Therefore, research is increasingly focused on the underlying stress response mechanisms of cotton. We first identified and cloned a novel gene encoding the 525 amino acids in cotton, namely GhWRKY6. qRT-PCR analysis indicated that GhWRKY6 was induced by NaCl, PEG 6000 and ABA. Analyses of germination rate and root length indicated that overexpression of GhWRKY6 in Arabidopsis resulted in hypersensitivity to ABA, NaCl, and PEG 6000. In contrast, the loss-of-function mutant wrky6 was insensitive and had slightly longer roots than the wild-type did under these treatment conditions. Furthermore, GhWRKY6 overexpression in Arabidopsis modulated salt- and drought-sensitive phenotypes and stomatal aperture by regulating ABA signaling pathways, and reduced plant tolerance to abiotic stress through reactive oxygen species (ROS) enrichment, reduced proline content, and increased electrolytes and malondialdehyde (MDA). The expression levels of a series of ABA-, salt- and drought-related marker genes were altered in overexpression seedlings. Virus-induced gene silencing (VIGS) technology revealed that down-regulation of GhWRKY6 increased salt tolerance in cotton. These results demonstrate that GhWRKY6 is a negative regulator of plant responses to abiotic stress via the ABA signaling pathway.

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