A Metalloprotease Homolog Venom Protein From a Parasitoid Wasp Suppresses the Toll Pathway in Host Hemocytes
文献类型： 外文期刊
作者： Lin, Zhe 1 ; Cheng, Yang 1 ; Wang, Rui-Juan 3 ; Du, Jie 1 ; Volovych, Olga 1 ; Li, Jian-Cheng 4 ; Hu, Yang 1 ; Lu, Zi-Yun 4 ;
作者机构： 1.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
3.Northwest A&F Univ, Coll Plant Protect, Dept Entomol, Yangling, Shaanxi, Peoples R China
4.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding, Peoples R China
关键词： venom; metalloprotease; toll pathway; Microplitis mediator; Helicoverpa armigera
期刊名称：FRONTIERS IN IMMUNOLOGY （ 影响因子：7.561； 五年影响因子：7.624 ）
ISSN： 1664-3224
年卷期： 2018 年 9 卷
页码：
收录情况： SCI
摘要： Parasitoid wasps depend on a variety of maternal virulence factors to ensure successful parasitism. Encapsulation response carried out by host hemocytes is one of the major host immune responses toward limiting endoparasitoid wasp offspring production. We found that VRF1, a metalloprotease homolog venom protein identified from the endoparasitoid wasp, Microplitis mediator, could modulate egg encapsulation in its host, the cotton bollworm, Helicoverpa armigera. Here, we show that the VRF1 proenzyme is cleaved after parasitism, and that the C-terminal fragment containing the catalytic domain enters host hemocytes 6 h post-parasitism. Furthermore, using yeast two-hybrid and pull-down assays, VRF1 is shown to interact with the H. armigera NF-kappa B factor, Dorsal. We also show that overexpressed of VRF1 in an H. armigera cell line cleaved Dorsal in vivo. Taken together, our results have revealed a novel mechanism by which a component of endoparasitoid wasp venom interferes with the Toll signaling pathway in the host hemocytes.
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