Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
文献类型： 外文期刊
作者： Liu Tianli 1 ; Wang Xifeng 1 ; Tian Zhenzhong 1 ; Wang Lixia 1 ; Zhang Xingxing 2 ; Qiao Jun 1 ; Meng Qingling 1 ; Gong S 1 ;
作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China
2.Xinjiang Acad Agr & Reclamat Silence, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Xinjiang, Peoples R China
3.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China
关键词： multi-epitope; fusion protein; serodiagnostic; antigen; cystic echinococcosis; indirect ELISA; sheep
期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.341； 五年影响因子：1.669 ）
ISSN： 0023-4001
年卷期： 2019 年 57 卷 1 期
页码：
收录情况： SCI
摘要： Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. Coll and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
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