Research Progress on Agrobacterium tumefaciens-based Transgenic Technology in Brassica rapa
文献类型： 外文期刊
作者： Li Guoliang 1 ; Yue Lixin 1 ; Li Fei 1 ; Zhang Shifan 1 ; Zhang Hui 1 ; Qian Wei 1 ; Fang Zhiyuan 1 ; Wu Jian 1 ; Wang Xiaow 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
关键词： Brassica rapa; Agrobacterium tumefaciens; transgenic; transformation
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：3.032； ）
ISSN： 2095-9885
年卷期： 2018 年 4 卷 3 期
页码：
收录情况： SCI
摘要： Brassica rapa L. is cultivated globally and consumed in many areas worldwide. Using the transgenic Agrobacterium-mediated transformation method, which is a reproducible and efficient technique, genes can be transferred into various B. rapa species. This review summarizes the processes involved in Agrobacterium-mediated transformation of B. rapa, including surface seed sterilization, co-cultivation with A. tumefaciens, induction of callus/shoot/root formation, and confirmation of transgenic plants. In addition, factors such as the Agrobacterium strain, plant genotype, explant age, transformation efficiency of the hybrid or inbred line, and the concentrations of N6-benzyl amino purine and naphthalene acetic acid, are discussed. And this review shows clearly how to do it, what to do, and what not to do in the transgenic Agrobacterium-mediated in Brassica rapa. The information presented here lays the foundation for a simple and efficient method that resolves existing problems and improves overall transgenic B. rapa production, thereby benefiting both basic and applied research.
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