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Distinct binding affinities of odorant-binding proteins from the natural predator Chrysoperla sinica suggest different strategies-to-hunt-prey

文献类型： 外文期刊

作者： Li, Zhao-Qun 1 ; Zhang, Shuai 1 ; Cai, Xiao-Ming 1 ; Luo, Jun-Yu 1 ; Dong, Shuang-Lin 1 ; Cui, Jin-Jie 1 ; Chen, Zong-Mao 1 ;

作者机构： 1.Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China

2.State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China

3.College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests (Nanjing Agricultural University), Ministry of Education, Nanjing 210095, China

关键词： Fluorescence binding assay; Lacewing; Tissue expression pattern; Odorant-binding protein

期刊名称：JOURNAL OF INSECT PHYSIOLOGY （ 影响因子：2.354； 五年影响因子：3.045 ）

ISSN： 0022-1910

年卷期： 2018 年 111 卷

页码：

收录情况： SCI

摘要： Chrysoperla sinica is an important natural predator of many notorious agricultural pests. Understanding its olfactory mechanism can help enhance the effectiveness of C. sinica in biological control. In the present study, we investigated the tissue expression patterns of 12 odorant-binding protein (OBP) genes from C. sinica (CsinOBPs). The results of quantitative real-time polymerase chain reaction (qPCR) showed that CsinOBP1, CsinOBP2, CsinOBP3, CsinOBP4, CsinOBP6, CsinOBP7, CsinOBP9, CsinOBP10, and CsinOBP12 were predominantly expressed in the antennae of both sexes, indicating their roles in olfaction. Additionally, the qPCR analysis revealed that the 12 CsinOBP genes had distinct expression patterns, while the motif-pattern investigation suggested that the OBPs had different ligands. The ligand-binding assay showed that CsinOBP1 and CsinOBP10 had broader binding spectra than did the other OBPs. Thus, CsinOBP1 was able to bind not only plant volatiles (such as farnesol, cis-3-hexenyl hexanoate, geranylacetone, beta-ionone, 2-tridecanone, and trans-nerolidol) but also the aphid alarm pheromone (E)-beta-farnesene. On the other hand, CsinOBP2 and CsinOBP6 exhibited relatively narrow binding spectra, only binding ethyl benzoate. The study also identified several compounds that can potentially be used to develop slow-release agents attracting C. sinica and to improve search strategies for insect pest control.

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