Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa (Medicago sativa L.) cultivars in response to aphid feeding
文献类型： 外文期刊
作者： Tu Xiong-bing 1 ; Zhao Hai-long 1 ; Zhang Ze-hua 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China
2.Shenyang Agr Univ, Coll Plant Protect, Shenyang 110161, Liaoning, Peoples R China
关键词： alfalfa cultivar resistance; aphids; transcriptomic; molecular mechanism
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2018 年 17 卷 11 期
页码：
收录情况： SCI
摘要： Plant breeding for resistance to agricultural pests is an essential element in the development of integrated crop management systems, however, the molecular and genetic mechanisms underlying resistance are poorly understood. In this pilot study, we conducted a transcriptomic analysis of a resistant (R) and susceptible (S) variety of alfalfa, with (+A) or without (-A) aphids (totally four treatments). We used the resistant cultivar Zhongmu 1 and the susceptible cultivar Soca. A total of 3 549 mRNAs were differentially expressed, of which 1738 up-regulated and 1 307 down-regulated genes were identified in S+A/S-A plants, while 543 up-regulated and 331 down-regulated genes were identified in the R+A/R-A plants. KEGG analysis mapped 112 and 546 differentially expressed genes to 8 and 17 substantially enriched pathways for Zhongmu 1 and Soca, respectively. Six shared pathways were linked to plant resistance traits, including phenylpropanoid biosynthesis associated with salicylic acid synthesis, and linoleic acid metabolism associated with both jasmonic acid and flavonoid biosynthesis. Ultimately, we proposed a preliminary regulatory mechanism of alfalfa cultivar resistance response to aphids feeding based on transcriptome analyses and published documents.
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