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An effective approach to cartilage regeneration using antler stem cell-conditioned medium

文献类型： 外文期刊

作者： Zhou, Jue 1 ; Xing, Baorui 2 ; Guo, Qianqian 3 ; Zhao, Jianwei 1 ; Li, Xunsheng 1 ; Wang, Datao 1 ; Yue, Zhigang 1 ; Lv, Jinpeng 1 ; Jiang, Yidi 1 ; Sun, Hongmei 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, 4899 Jingyue St, Changchun 130112, Jilin, Peoples R China

2.Jinan Stamotol Hosp, Jinan Key Lab Oral Tissue Regenerat, Shandong Prov Hlth Commiss Key Lab Oral Dis & Tiss, Jinan 250001, Shandong, Peoples R China

3.Changchun Sci Tech Univ, Inst Antler Sci & Prod Technol, Changchun 130112, Jilin, Peoples R China

关键词： Antler stem cells; Conditioned medium; Cell proliferation; Cartilage regeneration; Cartilage defect

期刊名称：SCIENTIFIC REPORTS （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN： 2045-2322

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Articular cartilage has a low capacity for self-regeneration. Therefore, stem cell therapy has been proposed and is gaining momentum. Antler can self-repair and its cartilaginous tissue can grow at an unprecedented rate (Up to 2 cm/day) and antler regeneration is based on antler stem cells. Therefore, we predicted that antler stem cells or their paracrine factors might be a good source for promoting cartilage repair. In this study, we prepared conditioned medium from antler stem cells (ASC-CM) and evaluated its effect. We implanted the medium into rat cartilage defects and assessed its capacity to promote cartilage repair. The protein composition of ASC-CM was analyzed using via DIA assay. ASC-CM can strongly promote chondrocyte proliferation in vitro; it significantly up-regulates the expression of chondrogenesis-related genes (Aggrecan, Col II, and Sox-9) and promotes glycosaminoglycan formation and type II collagen deposition in cartilaginous tissue; meanwhile up-regulate the apoptosis suppressor gene NAMPT and down-regulate apoptosis gene BAX. In vivo, cartilage defect in rats was significantly repaired using ASC-CM. The compositions of ASC-CM were got and some key proteins such as S100A4 were identified by bioinformatics analysis. The ASC-CM can promote the proliferation of rat-chondrocytes, maintain the chondrocyte phenotype, and inhibit apoptosis of rat-chondrocytes. Therefore, it can promote cartilage repair.

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