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Biosynthesis of Cytosporones in Leotiomycetous Filamentous Fungi

文献类型： 外文期刊

作者： Li, Li 1 ; Zhong, Weimao 1 ; Liu, Hang 1 ; Espinosa-Artiles, Patricia 1 ; Xu, Ya-ming 1 ; Wang, Chen 1 ; Verdugo Robles, Jose Manuel 1 ; Paz, Tiago Antunes 5 ; Inacio, Marielle Cascaes 1 ; Chen, Fusheng 6 ; Xu, Yuquan 4 ; Gunatilaka, A. A. Leslie 1 ; Molnar, Istvan 1 ;

作者机构： 1.Univ Arizona, Southwest Ctr Nat Prod Res, Tucson, AZ 85719 USA

2.Yangtze Univ, Coll Life Sci, Jingzhou 434025, Peoples R China

3.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA USA

4.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

5.Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, Brazil

6.Guizhou Normal Univ, Sch Life Sci, Guiyang 550025, Peoples R China

7.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

8.VTT Tech Res Ctr Finland Ltd, Espoo 02150, Finland

期刊名称：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY （ 影响因子：15.0； 五年影响因子：15.1 ）

ISSN： 0002-7863

年卷期： 2024 年 146 卷 9 期

页码：

收录情况： SCI

摘要： Polyketides with the isochroman-3-one pharmacophore are rare among fungal natural products as their biosynthesis requires an unorthodox S-type aromatic ring cyclization. Genome mining uncovered a conserved gene cluster in select leotiomycetous fungi that encodes the biosynthesis of cytosporones, including isochroman-3-one congeners. Combinatorial biosynthesis in total biosynthetic and biocatalytic formats in Saccharomyces cerevisiae and in vitro reconstitution of key reactions with purified enzymes revealed how cytosporone structural and bioactivity diversity is generated. The S-type acyl dihydroxyphenylacetic acid (ADA) core of cytosporones is assembled by a collaborating polyketide synthase pair. Thioesterase domain-catalyzed transesterification releases ADA esters, some of which are known Nur77 modulators. Alternatively, hydrolytic release allows C6 hydroxylation by a flavin-dependent monooxygenase, yielding a trihydroxybenzene moiety. Reduction of the C9 carbonyl by a short chain dehydrogenase/reductase initiates isochroman-3-one formation, affording cytosporones with cytotoxic and antimicrobial activity. Enoyl di- or trihydroxyphenylacetic acids are generated as shunt products, while isocroman-3,4-diones are formed by autoxidation. The cytosporone pathway offers novel polyketide biosynthetic enzymes for combinatorial synthetic biology to advance the production of "unnatural" natural products for drug discovery.

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