Advancing in molecular taxonomy: DNA barcode, a tool to resolve taxonomy and phylogeny of family euphorbiaceous
文献类型： 外文期刊
作者： Alyas, Tahira 1 ; Shaheen, Shabnum 1 ; Rahman, Khursheed Ur 2 ; Ahmad, Jalil 3 ; Kamal, Asif 4 ; Aziz, Ejaz 5 ; Albasher, Gadah 6 ; Razak, Sarah Abdul 7 ; Zaman, Wajid 8 ;
作者机构： 1.Lahore Coll Women Univ, Dept Bot, Lahore 54000, Pakistan
2.Hazara Univ, Dept Bot, Mansehra 21300, Pakistan
3.Chinese Acad Agr Sci, Inst Vegetable & Flowers, Beijing 100081, Peoples R China
4.Islamabad Career Coll, Dept Bot, Kiyani Rd Bharakahu, Islamabad, Pakistan
5.GDC Khanpur, Dept Bot, Haripur 22650, Khyber Pakhthun, Pakistan
6.King Saud Univ, Coll Sci, Dept Zool, Riyadh 11451, Saudi Arabia
7.Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur 50603, Malaysia
8.Yeungnam Univ, Dept Life Sci, Gyongsan 38541, South Korea
关键词： Euphorbiaceae; DNA barcoding; Phylogenetic study; Taxonomy
期刊名称：GENETIC RESOURCES AND CROP EVOLUTION
ISSN： 0925-9864
年卷期： 2025 年 72 卷 4 期
页码：
收录情况： SCI
摘要： Euphorbiaceous plants are economic important such as castor oil, rubber source, nut, ornamental as well as herbal medicines with significant values of phytotoxin. In the current research, the diversity among some selected members of the family Euphorbiaceae collected from different areas of Pakistan was observed by utilizing molecular markers. DNA barcoding of the collected specimens was carried out for the analysis of the phylogeny of the various plant resources comprising of short, gene standard sequences in a precise, rapid and feasible manner. Three barcodes (matK, rbcL & nrITS) were utilized in combination for 22 different plant samples collected from different areas of Pakistan. All three barcodes were individually helpful in identification at families' levels of taxon among collected species. However, barcode matK and nrITS when used in combination were found helpful for the identification up to genus level and all three barcodes when used in combination lead to the correct identification of specimens up to species level.Trial registration ISRCTN.org Identifier: ISRCTN60048867. FIRST-ABC was prospectively registered with the ISRCTN Registry on 19 June 2019.
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