文献类型： 外文期刊

作者： Wang, Aolin ¹ ; Shang, Zhaoyue ¹ ; Jiang, Ru ¹ ; Zhang, Meihui ¹ ; Wang, Jifeng ¹ ; Li, Hongfu ¹ ; Zhang, Bin ¹ ; Tang, Huarong ¹ ; Xu, Fei ⁴ ; Hu, Xiaoping ² ; Liu, Wei ¹ ; Fan, Jieru ¹ ; Zhou, Yilin ¹ ; West, Jonathan S. ⁵ ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

2.Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Resistance & High Effici, Yangling 712100, Peoples R China

3.Xinjiang Agr Univ, Coll Agron, Key Lab Pest Monitoring & Safety Control Crops & F, Urumqi 830052, Peoples R China

4.Henan Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops Southern, Minist Agr & Rural Affairs Peoples Republ China, Zhengzhou 450002, Peoples R China

5.Rothamsted Res, Harpenden AL5 2JQ, England

关键词： airborne inoculum; fungal diseases of wheat; monitoring; multiplex real-time PCR; spore quantification; spore traps

期刊名称：PLANT DISEASE （ 影响因子：4.4； 五年影响因子：4.8 ）

ISSN： 0191-2917

年卷期： 2025 年 109 卷 2 期

页码：

收录情况： SCI

摘要： Common wheat (Triticum aestivum L.) production in China is challenged by stripe (yellow) rust, powdery mildew, and Fusarium head blight. Airborne inoculum of these pathogens is the causative driver of disease epidemics. Thus, monitoring of airborne inoculum on such fungal diseases is expected to provide some reliable estimations of disease development, especially by targeting multiple diseases simultaneously. This paper reports the development of a new practical qPCR-based method coupled with spore trapping to quantify simultaneously airborne inoculum of Puccinia striiformis f. sp. tritici (Pst), Blumeria graminis f. sp. tritici (Bgt), as well as Fusarium graminearum (Fg) and F. asiaticum and discusses its potential use in disease-risk warnings. The technique can detect DNA of Pst and Bgt at quantities as low as 0.2 pg, and 2 pg for Fg (i.e., representing 0.65 urediniospores, 1.18 conidia, and 10 macroconidia, respectively), and neither T. aestivum DNA nor DNA of other common wheat pathogens were amplified. Linear relationships were produced between the number of spores on tapes determined by qPCR and conventional microscopy, with a small variation (R2 value 0.97 to 0.99 depending on pathogen species). The daily concentrations of spores of the three pathogens were monitored using a Burkard 7-day recording spore trap, and the airborne spores were collected from a field near Langfang City, Hebei Province, China. The patterns of spore concentration dynamics in the air determined by triplex qPCR were close to those counted by conventional microscopy in a duplicated subsample. The developed assay can be an alternative to conventional microscopy to process large samples. This will improve monitoring power by providing timely risk warning information to growers regarding the timing of fungicide applications.