Cytokinesis-Defective 1 (CYT1) Positively Regulates Plant Antiviral Immunity by Promoting Callose Deposition and Ascorbic Acid Biosynthesis
文献类型： 外文期刊
作者： Jiang, Xue 1 ; Wang, Yuting 1 ; Zhao, Yu 1 ; Korxeelor, Xayvangye 1 ; Fan, Wenqian 1 ; Fan, Xinyue 1 ; Li, Yong 2 ; Wu, Xiaoxia 3 ; Zhou, Xueping 4 ; Li, Fangfang 4 ; Wu, Xiaoyun 1 ; Ji, Weiqin 1 ; Cheng, Xiaofei 1 ;
作者机构： 1.Northeast Agr Univ, Coll Plant Protect, Harbin, Heilongjiang, Peoples R China
2.Northeast Agr Univ, Sch Life Sci, Harbin, Heilongjiang, Peoples R China
3.Northeast Agr Univ, Coll Agr, Harbin, Heilongjiang, Peoples R China
4.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China
关键词： ascorbic acid; callose; CYT1; NIb; TuMV
期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.6 ）
ISSN： 1464-6722
年卷期： 2025 年 26 卷 7 期
页码：
收录情况： SCI
摘要： NUCLEAR INCLUSION B (NIb), the RNA-dependent RNA polymerase (RdRp) of potyviruses, plays a critical role in both viral replication and suppression of host antiviral immunity. However, the mechanisms by which NIb suppresses host immunity remain poorly understood. In this study, we used affinity purification-mass spectrometry to identify host factors interacting with NIb encoded by turnip mosaic virus (TuMV). We identified 57 potential NIb-interacting host factors, including mannose-1-phosphate guanylyltransferase CYTOKINESIS-DEFECTIVE 1 (CYT1). Virus infectivity assays showed that TuMV infection was significantly attenuated in Nicotiana benthamiana leaves transiently expressing CYT1 and in transgenic Arabidopsis overexpressing CYT1. Exogenous application of ascorbic acid (AsA) and inhibition of N-linked glycosylation reduced virus infection. Furthermore, overexpression of CYT1 induced callose deposition, and inhibition of callose synthesis enhanced virus infection. We also demonstrated that NIb interacts with the C-terminal domain of CYT1, affects its cytosolic distribution, and inhibits AsA accumulation. These findings suggest that CYT1 positively regulates plant antiviral immunity by promoting callose deposition and ascorbic acid biosynthesis.
- 相关文献
作者其他论文 更多>>
-
Using Quantitative Trait Locus Mapping and Genomic Resources to Improve Breeding Precision in Peaches: Current Insights and Future Prospects
作者：Hayat, Umar;Ke, Cao;Wang, Lirong;Zhu, Gengrui;Fang, Weichao;Wang, Xinwei;Chen, Changwen;Li, Yong;Wu, Jinlong;Hayat, Umar;Ke, Cao
关键词：peach; QTL; marker-assisted breeding; genomic selection; CRISPR/Cas9; fruit shape
-
Largemouth bronze gudgeon (Coreius guichienoti) responds positively to liver damage induced by high feeding rates via enhancing self-repair capability
作者：Chen, Pei;Jiang, Wei;Yang, Jing;Zhao, Yu;Qu, Huantao;Liang, Xiaofang;Lei, Chao;Chen, Pei;Qu, Huantao
关键词：Largemouth bronze gudgeon; Feeding rate; Lipid metabolism; Inflammation and apoptosis; Immunity
-
RNA modifications in plant biotic interactions
作者：Ge, Linhao;Pan, Fuan;Jia, Mingxuan;He, Hao;Shan, Hongying;Zhou, Xueping;Li, Fangfang;Pan, Fuan;Zhou, Xueping;Pott, Delphine M.;Wang, Aiming
关键词：RNA modifications; N6-methyladenosine; m6A; m6A core components; plant biotic stress; plant- microbe interactions; plant-insect interactions
-
Soybean stay-green associated geminivirus: A serious threat to soybean production in China
作者：He, Hao;Li, Hao;Zhou, Xueping;Li, Fangfang;He, Hao;Cui, Xiaoyan;Wang, Yaqin;Zhou, Xueping;Xu, Yi
关键词：Soybean; Soybean stay-green associated geminivirus; (SoSGV); Soybean stay-green syndrome; Viral transmission; Viral epidemiology
-
Multiplex lateral flow immunoassay for simultaneous detection and specific discrimination of Xoo and Xoc in rice plants
作者：Zhang, Cui;Zhang, Xi;Liu, Nairu;Dong, Jie;Mao, Weijia;Zhou, Xueping;Wu, Jianxiang;Zhang, Cui;Wu, Jianxiang;Liu, Zhaoli;Zhou, Xueping
关键词：Multiplex lateral flow immunoassay; Simultaneous detection; AuNPs; Xoo; Xoc
-
Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein
作者：Liu, Qiang;Niu, Xiaoxia;Jiang, Lingling;Zhang, Gang;Wang, Pu;Zhang, Sinong;Gao, Weifeng;Guo, Huichen;Wang, Yujiong;Li, Yong;Guo, Huichen
关键词：bovine coronavirus; N protein; eukaryotic expression; indirect ELISA; antibody detection
-
Deciphering intricate plant-virus interactions: Potyvirids orchestrate protein posttranslational modifications to regulate pathogenicity
作者：Kamran, Ali;Hussain, Muhammad Dilshad;Khan, Mehran;Yang, Sanwei;Xie, Xin;Farooq, Tahir;Li, Fangfang;Li, Xiangyang;Li, Xiangyang
关键词：potyvirids; post-translational modifications; phosphorylation; SUMOylation; ubiquitination; O-GlcNAcylation
