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Breakthrough in CRISPR/Cas system: Current and future directions and challenges

文献类型: 外文期刊

作者: Ali, Ahmad 1 ; Zafar, Muhammad Mubashar 2 ; Farooq, Zunaira 3 ; Ahmed, Syed Riaz 4 ; Ijaz, Aqsa 4 ; Anwar, Zunaira 4 ; Abbas, Huma 5 ; Tariq, Muhammad Sayyam 4 ; Tariq, Hala 6 ; Mustafa, Mahwish 7 ; Bajwa, Mubasher Hussain 8 ; Shaukat, Fiza 7 ; Razzaq, Abdul 2 ; Maozhi, Ren 2 ;

作者机构: 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, Anyang 455000, Peoples R China

3.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Key Lab Biol & Genet Improvement Soybean Gen,Minis, Nanjing, Peoples R China

4.Pakistan Inst Engn & Appl Sci PIEAS, Nucl Inst Agr & Biol Coll NIAB C, Nilore, Pakistan

5.Univ Agr Faisalabad, Dept Plant Pathol, Faisalabad, Pakistan

6.Univ Agr Faisalabad, Inst Soil & Environm Sci, Faisalabad, Pakistan

7.Univ Agr Faisalabad, Ctr Agr Biochem & Biotechnol, Faisalabad, Pakistan

8.Univ Agr Faisalabad, Inst Anim & Dairy Sci, Faisalabad, Pakistan

9.Univ Lahore, Inst Mol Biol & Biotechnol, Lahore 54792, Pakistan

10.Chinese Acad Agr Sci, Inst Urban Agr, Chengdu, Peoples R China

关键词: base editing; CRISPR-Cas; genome editing; multiplex editing; prime editing; TALENs; ZFNs

期刊名称:BIOTECHNOLOGY JOURNAL ( 影响因子:4.7; 五年影响因子:4.5 )

ISSN: 1860-6768

年卷期: 2023 年

页码:

收录情况: SCI

摘要: Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists to develop desirable varieties. This technology involves inducing site-specific DNA perturbations that can be repaired through DNA repair pathways. GE products currently include CRISPR-associated nuclease DNA breaks, prime editors generated DNA flaps, single nucleotide-modifications, transposases, and recombinases. The discovery of double-strand breaks, site-specific nucleases (SSNs), and repair mechanisms paved the way for targeted GE, and the first-generation GE tools, ZFNs and TALENs, were successfully utilized in plant GE. However, CRISPR-Cas has now become the preferred tool for GE due to its speed, reliability, and cost-effectiveness. Plant functional genomics has benefited significantly from the widespread use of CRISPR technology for advancements and developments. This review highlights the progress made in CRISPR technology, including multiplex editing, base editing (BE), and prime editing (PE), as well as the challenges and potential delivery mechanisms.

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