Beta-carotene regulates the biological activity of EGF in IEC6 cells by alleviating the inflammatory process
文献类型： 外文期刊
作者： Wang Guoxia 1 ; Yang Yu 2 ; Zhang, Shuai 1 ; Lan Hainan 1 ; Xin Zheng 1 ;
作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China
2.Chinese Acad Agr Sci, Beijing Inst Anim Husb & Vet Med, Beijing, Peoples R China
关键词： Inflammation; IEC6 cell; EGF; EGFR
期刊名称：CELL CYCLE （ 影响因子：5.173； 五年影响因子：4.948 ）
ISSN： 1538-4101
年卷期： 2022 年 21 卷 16 期
页码：
收录情况： SCI
摘要： Epidermal growth factor (EGF) has many important biological functions. It plays an important role in regulating the growth, survival, migration, apoptosis, proliferation, and differentiation of intestinal tissues and cells. However, until now, the effect of inflammation on the biological activity of EGF in intestinal cells or tissues is still unclear. For this reason, in the current research, we have conducted a detailed study on this issue. Using the rat small intestinal crypt epithelial cell line (IEC6) was used as an in vitro model, and Confocal laser scanning microscope (CLSM), Flow cytometry (FCM), Indirect immunofluorescence assay (IFA), Western-blotting (WB), and Quantitative real-time RT-PCR (QRT-PCR) methods were used to explore the effects of inflammation on EGF/EGFR biological activity and signal transduction profiles. We found that the EGF/EGFR nuclear signal almost disappeared in the inflammatory state, and the phosphorylation levels of EGFR, AKT, and STAT3 were all significantly down-regulated. In addition, we also studied the effect of beta-carotene on the biological activity of EGF, and found that when cells were pretreated with beta-carotene, the cellular behavior, biological activity, and nuclear signal of EGF/EGFR under inflammation stimulation were partially restored. In summary, the current study shows that inflammation can disrupt EGF/EGFR-mediated signaling in IEC6 cells, suggesting that inflammation negatively regulates the biological activity of EGF/EGFR. Furthermore, we found that beta-carotene not only attenuated lipopolysaccharide (LPS)-induced inflammation but also partially restored the biological activity of EGF in IEC6 cells, laying a solid foundation for studying the biological functions of EGF and beta-carotene.
- 相关文献
作者其他论文 更多>>
-
Identification and field verification of the aggregation pheromone components produced by male Holotrichia parallela Motschulsky (Coleoptera: Scarabaeidae)
作者：Qu, Ya-Fei;Li, Xue;Dong, Huan-Huan;Wang, Wen-Jie;Li, Xiao-Feng;Zhang, Shuai;Cao, Yazhong;Yin, Jiao;Li, Kebin;Feng, Honglin
关键词：Holotrichia parallela; aggregation pheromone; pentadecyl acetate; cis-13-docosenol; behenic acid
-
Genome-wide analyses of glutathione S-transferase gene family and expression profiling among three haplotypes Aphis gossypii
作者：Zhang, Yaling;Farhan, Muhammad;Yang, Hanjing;Zhao, Jun;Zhang, Shuai;Ma, Xiaoyan
关键词：Cotton-melon aphid; Population differentiation; Host adaptation; Pesticide resistance
-
A near-complete genome reveals the population evolution of the cotton-melon aphid Aphis gossypii
作者：Jing, Tianxing;Yang, Jin;Pan, Jilong;Liu, Xiaoning;Yang, Xinyi;Farhan, Muhammad;Su, Honghua;Zhang, Shuai;Ma, Xiaoyan
关键词：Aphid; Haplotype; Host adaption; Comparative genomics; Horizontal gene transfer
-
Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol
作者：Bai, Shanshan;Ge, Yifei;Yao, Dongtan;Wang, Yifan;Tan, Jinfang;Peng, Yutao;Jiang, Xiaoqian;Bai, Shanshan;Tan, Jinfang;Zhang, Shuai
关键词：
-
Preliminary Investigation on Resistance of Beckmannia syzigachne to Clodinafop-Propargyl and Mesosulfuron-Methyl from China
作者：Peng, Licun;Li, Xiangju;Guo, Xiaotong;Li, Zheng;Chen, Jingchao;Wei, Shouhui;Cui, Hailan;Zhang, Shuai;Guo, Xiaotong
关键词：
Beckmannia syzigachne ; clodinafop-propargyl; mesosulfuron-methyl; resistance; mutation -
Adaptability of Aphis gossypii glover to different Capsicum annuum varieties
作者：Yang, Jin;Bang, Hao;Yang, Hanjing;Zhao, Jun;Farhan, Muhammad;Zhang, Shuai;Ma, Xiaoyan
关键词：adaptability;
Aphis gossypii glover; biotype;Capsicum annuum ; life table -
Establishment of goat infection model of the peste des petits ruminants virus isolated in China for vaccine efficacy evaluation
作者：Wang, Xue;Chen, Hefeng;Zhang, Xianfeng;Wu, Zhengshuang;Zhang, Shuai;Shuai, Lei;Wang, Lulu;Li, Weijie;Wang, Jinliang;Liu, Wenxing;Wang, Xijun;Wen, Zhiyuan;Ge, Jinying;Guan, Yuntao;He, Xijun;Chen, Weiye;Bu, Zhigao;Bu, Zhigao
关键词：peste des petits ruminants virus; Chinese isolate; goat infection model; vaccine efficacy evaluation
