文献类型： 外文期刊

作者： Cao Su-mei ¹ ; Li Ting-ting ¹ ; Shao Yu-xin ² ; Zhao Yu-zhen ⁴ ; Zhang Li-yang ² ; Lu Lin ² ; Zhang Ri-jun ³ ; Hou Shui-sheng ² ; Liao Xiu-dong ² ; Luo Xu-gang ¹ ; Wang Run-lian ⁴ ;

作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Poultry Mineral Nutr Lab, Yangzhou 225000, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Mineral Nutr Res Div, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

3.China Agr Univ, Coll Anim Sci & Technol, Lab Feed Biotechnol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

4.Guangdong Ocean Univ, Dept Anim Sci, Zhanjiang 524088, Peoples R China

关键词： broiler; tibial osteoblast; primary culture; vitality; mineralization

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2023 年 22 卷 2 期

页码：

收录情况： SCI

摘要： Osteoblasts are considered as a major factor contributing to bone development and mineralization, however, few studies have been done to establish and evaluate the primary cultured tibial osteoblast model of broiler chicks. Therefore, in the present study, two experiments were conducted to establish and evaluate the primary cultured tibial osteoblast model of broiler chicks. In experiment 1, osteoblasts were isolated from the tibia of one-day-old Arbor Acre male broiler chicks using the explant method and identified through the cell morphology, alkaline phosphatase (ALP) and alizarin red staining. Experiment 2 was carried out to evaluate the vitality and mineralization of primary cultured tibial osteoblasts of broilers on days 4, 8, 12, 16, 20, 24, 28 and 32 after incubation, respectively. The results from experiment 1 demonstrated that primary cultured tibial osteoblasts of broilers showed a spindle-shaped, triangular or polygonal morphology. More than 95% of the cells were stained blue-black after ALP staining, and mineralized nodules were formed after 4 days of continuous incubation. In experiment 2, lactate dehydrogenase (LDH) activity stayed at a relatively stabilized level although incubation time affected ( P=0.0012) it during the whole culture period. Additionally, incubation time affected (P <= 0.0001) the number and proportion of the area of mineralized nodules. They increased linearly and quadratically (P<0.04) with the increase of incubation time, and remained at a stabilized level from 24 to 32 days of incubation. The estimates of the optimal incubation time were 17 and 26 days based on the best fitted broken-line or quadratic models (P<0.0001) of the number and proportion of the area of mineralized nodules, respectively. These results indicate that the primary cultured tibial osteoblast model of broilers has been established successfully by the explant method, and it showed typical osteoblast morphology and characteristics of ALP activity and mineralization, and could maintain a relatively stabilized vitality from 4 to 32 days of incubation; and the optimal incubation time of primary tibial osteoblasts was 17 to 26 days. Therefore, it could be used to further study the underlying mechanisms of bone development and mineralization of broiler chicks.