文献类型： 外文期刊

作者： Wang, Jianzhong ¹ ; Liu, Lina ⁴ ; Zong, Xianchun ¹ ; Wang, Chunliu ¹ ; Zhu, Guangmei ¹ ; Yang, Guilian ¹ ; Jiang, Yanlong ¹ ; Yang, Wentao ¹ ; Huang, Haibin ¹ ; Shi, Chunwei ¹ ; Zeng, Yan ¹ ; Wang, Nan ¹ ; Cao, Xin ¹ ; Wang, Chunfeng ¹ ; Feng, Na ⁴ ;

作者机构： 1.Jilin Agr Univ, Coll Vet Med, Changchun, Peoples R China

2.Jilin Agr Univ, Jilin Prov Engn Res Ctr Anim Probiot, Jilin Prov Key Lab Anim Microecol & Hlth Breeding, Changchun, Peoples R China

3.Jilin Agr Univ, Engn Res Ctr Microecol Vaccines Drugs Major Anim D, Minist Educ, Changchun, Peoples R China

4.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun, Peoples R China

5.Jilin Univ, Coll Vet Med, Changchun, Peoples R China

关键词： bacterium-like particles; canine distemper virus; subunit vaccine; surface display technology; immune response

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2024 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Canine distemper virus (CDV) poses a severe threat to both domesticated and wild animals, including multiple carnivores. With the continued expansion of its host range, there is an urgent need for the development of a safer and more effective vaccine. In this study, we developed subunit vaccines based on a bacterium-like particle (BLP) delivery platform containing BLPs-F and BLPs-H, which display the CDV F and H glycoprotein antigens, respectively, using the antigen-protein anchor fusions produced by a recombinant baculovirus insect cell expression system. The combination of BLPs-F and BLPs-H (CDV-BLPs), formulated with colloidal manganese salt [Mn jelly (MnJ)] adjuvant, triggered robust CDV-specific antibody responses and a substantial increase in the number of interferon gamma (IFN-gamma)-secreting CD4+ and CD8+ T cells in mice. Dogs immunized intramuscularly with this vaccine not only produced CDV-specific IgG but also displayed elevated concentrations of IFN-gamma and interleukin 6 in their serum, along with an increase of the CD3+CD4+ and CD3+CD8+ T cell subsets. Consequently, this heightened immune response provided effective protection against disease development and reduced viral shedding levels following challenge with a virulent strain. These findings suggest that this BLP-based subunit vaccine has the potential to become a novel canine distemper vaccine.IMPORTANCEMany sensitive species require a safe and effective distemper vaccine. Non-replicating vaccines are preferred. We constructed subunit particles displaying canine distemper virus (CDV) antigens based on a bacterium-like particle (BLP) delivery platform. The CDV-BLPs formulated with theMn jelly adjuvant induced robust humoral and cell-mediated immune responses to CDV in mice and dogs, thereby providing effective protection against a virulent virus challenge. This work is an important step in developing a CDV subunit vaccine. Many sensitive species require a safe and effective distemper vaccine. Non-replicating vaccines are preferred. We constructed subunit particles displaying canine distemper virus (CDV) antigens based on a bacterium-like particle (BLP) delivery platform. The CDV-BLPs formulated with theMn jelly adjuvant induced robust humoral and cell-mediated immune responses to CDV in mice and dogs, thereby providing effective protection against a virulent virus challenge. This work is an important step in developing a CDV subunit vaccine.