Cloning and characterization of repetitive sequences and development of SCAR markers specific for the P genome of Agropyron cristatum
文献类型： 外文期刊
作者： Wu, M. 1 ; Zhang, J. P. 1 ; Wang, J. C. 1 ; Yang, X. M. 1 ; Gao, A. N. 1 ; Zhang, X. K.; Liu, W. H. 1 ; Li, L. H. 1 ;
作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, NFCRI, Inst Crop Sci, Beijing 100081, Peoples R China
2.NW A&F Univ, Coll Agron, Yangling 712100, Shanxi Province, Peoples R China
3.NW A&F Univ, Coll Agron, Yangling 712100, Shanxi Pro
期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）
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收录情况： SCI
摘要： In previous studies, we successfully transferred the P genome of Agropyron cristatum into wheat using wide hybridization methods. In the current investigation, repetitive sequences were cloned and DNA markers specific for the P genome of A. cristatum were developed. Three P genome-specific markers, designated OPX07-1036, OPX11-817 and OPC05-1539, were identified and isolated using random amplified polymorphic DNAs. The three markers were successfully amplified in all tested materials that contained Agropyron chromatin, such as Agropyron itself and wheat-Agropyron addition lines. These RAPD markers were converted into SCAR markers to be used in detection of P genome chromatin in wheat. In situ probing with fluorescent-labeled marker DNA has shown that they are distributed in all arms of Agropyron hence they will be useful in a variety of studies on introgressions of the P-genome chromatin into wheat.
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